The involvement of phospholipase A2 in ethanol-induced gastric muscle contraction

Sang Soo Sim, Jae Chun Choi, Do Sik Min, Duck Joo Rhie, Shin Hee Yoon, Sang June Hahn, Chang Jong Kim, Myung Suk Kim, Yang Hyeok Jo

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9 Citations (Scopus)

Abstract

To understand the underlying mechanism of ethanol in tonic contraction, the effect of ethanol on phospholipase A2 and phospholipase C activities and the effects of phospholipase inhibitors on ethanol-induced contraction of cat gastric smooth muscle were tested. Circular muscle strips (2.0 × 0.2 cm) obtained from the fundus of cat stomach were used to measure isometric contraction. Ethanol elicited tonic contraction and activated phospholipase A2 activity in a dose-dependent manner. Phospholipase A2 inhibitors, manoalide (0.1-10 μM) and oleyloxyethyl phosphorylcholine (1-10 μM), significantly inhibited ethanol-induced contraction. Furthermore, 342 mM ethanol-induced contraction was significantly inhibited by cyclooxygenase inhibitors, ibuprofen (10-100 μM) and indomethacin (10-100 μM), but not by lipoxygenase inhibitors. On the other hand, phospholipase C inhibitors had no effect on ethanol-induced contraction, indicating that phospholipase C is not involved in ethanol-induced contraction. It is suggested from the above results that ethanol-induced contraction in cat gastric smooth muscle is, in part, mediated by phospholipase A2 and cyclooxygenase pathways.

Original languageEnglish
Pages (from-to)281-285
Number of pages5
JournalEuropean Journal of Pharmacology
Volume413
Issue number2-3
DOIs
Publication statusPublished - 2001 Feb 16

Bibliographical note

Funding Information:
The authors wish to acknowledge the financial support of the Korea Research Foundation made in the program year of (1998).

All Science Journal Classification (ASJC) codes

  • Pharmacology

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