Abstract
To understand the underlying mechanism of ethanol in tonic contraction, the effect of ethanol on phospholipase A2 and phospholipase C activities and the effects of phospholipase inhibitors on ethanol-induced contraction of cat gastric smooth muscle were tested. Circular muscle strips (2.0 × 0.2 cm) obtained from the fundus of cat stomach were used to measure isometric contraction. Ethanol elicited tonic contraction and activated phospholipase A2 activity in a dose-dependent manner. Phospholipase A2 inhibitors, manoalide (0.1-10 μM) and oleyloxyethyl phosphorylcholine (1-10 μM), significantly inhibited ethanol-induced contraction. Furthermore, 342 mM ethanol-induced contraction was significantly inhibited by cyclooxygenase inhibitors, ibuprofen (10-100 μM) and indomethacin (10-100 μM), but not by lipoxygenase inhibitors. On the other hand, phospholipase C inhibitors had no effect on ethanol-induced contraction, indicating that phospholipase C is not involved in ethanol-induced contraction. It is suggested from the above results that ethanol-induced contraction in cat gastric smooth muscle is, in part, mediated by phospholipase A2 and cyclooxygenase pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 281-285 |
| Number of pages | 5 |
| Journal | European Journal of Pharmacology |
| Volume | 413 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 2001 Feb 16 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
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The involvement of phospholipase A2 in ethanol-induced gastric muscle contraction. / Sim, Sang Soo; Choi, Jae Chun; Min, Do Sik; Rhie, Duck Joo; Yoon, Shin Hee; Hahn, Sang June; Kim, Chang Jong; Kim, Myung Suk; Jo, Yang Hyeok.
In: European Journal of Pharmacology, Vol. 413, No. 2-3, 16.02.2001, p. 281-285.Research output: Contribution to journal › Article
TY - JOUR
T1 - The involvement of phospholipase A2 in ethanol-induced gastric muscle contraction
AU - Sim, Sang Soo
AU - Choi, Jae Chun
AU - Min, Do Sik
AU - Rhie, Duck Joo
AU - Yoon, Shin Hee
AU - Hahn, Sang June
AU - Kim, Chang Jong
AU - Kim, Myung Suk
AU - Jo, Yang Hyeok
PY - 2001/2/16
Y1 - 2001/2/16
N2 - To understand the underlying mechanism of ethanol in tonic contraction, the effect of ethanol on phospholipase A2 and phospholipase C activities and the effects of phospholipase inhibitors on ethanol-induced contraction of cat gastric smooth muscle were tested. Circular muscle strips (2.0 × 0.2 cm) obtained from the fundus of cat stomach were used to measure isometric contraction. Ethanol elicited tonic contraction and activated phospholipase A2 activity in a dose-dependent manner. Phospholipase A2 inhibitors, manoalide (0.1-10 μM) and oleyloxyethyl phosphorylcholine (1-10 μM), significantly inhibited ethanol-induced contraction. Furthermore, 342 mM ethanol-induced contraction was significantly inhibited by cyclooxygenase inhibitors, ibuprofen (10-100 μM) and indomethacin (10-100 μM), but not by lipoxygenase inhibitors. On the other hand, phospholipase C inhibitors had no effect on ethanol-induced contraction, indicating that phospholipase C is not involved in ethanol-induced contraction. It is suggested from the above results that ethanol-induced contraction in cat gastric smooth muscle is, in part, mediated by phospholipase A2 and cyclooxygenase pathways.
AB - To understand the underlying mechanism of ethanol in tonic contraction, the effect of ethanol on phospholipase A2 and phospholipase C activities and the effects of phospholipase inhibitors on ethanol-induced contraction of cat gastric smooth muscle were tested. Circular muscle strips (2.0 × 0.2 cm) obtained from the fundus of cat stomach were used to measure isometric contraction. Ethanol elicited tonic contraction and activated phospholipase A2 activity in a dose-dependent manner. Phospholipase A2 inhibitors, manoalide (0.1-10 μM) and oleyloxyethyl phosphorylcholine (1-10 μM), significantly inhibited ethanol-induced contraction. Furthermore, 342 mM ethanol-induced contraction was significantly inhibited by cyclooxygenase inhibitors, ibuprofen (10-100 μM) and indomethacin (10-100 μM), but not by lipoxygenase inhibitors. On the other hand, phospholipase C inhibitors had no effect on ethanol-induced contraction, indicating that phospholipase C is not involved in ethanol-induced contraction. It is suggested from the above results that ethanol-induced contraction in cat gastric smooth muscle is, in part, mediated by phospholipase A2 and cyclooxygenase pathways.
UR - http://www.scopus.com/inward/record.url?scp=0035895541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035895541&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(01)00753-1
DO - 10.1016/S0014-2999(01)00753-1
M3 - Article
C2 - 11226404
AN - SCOPUS:0035895541
VL - 413
SP - 281
EP - 285
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -