The maintenance of free-living amoebae by cryopreservation.

S. A. Seo, Taisoon Yong, K. Im

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall 0.7 degree C/min) than by fast cooling (overall 1.3 degrees C/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophozoites. The survival rate was 2-39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.

Original languageEnglish
Pages (from-to)151-153
Number of pages3
JournalKisaengch'unghak chapchi. The Korean journal of parasitology
Volume30
Issue number2
DOIs
Publication statusPublished - 1992 Jan 1

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Trophozoites
Amoeba
Cryopreservation
Maintenance
Dimethyl Sulfoxide
Glycerol
Suspensions
Nitrogen

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

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The maintenance of free-living amoebae by cryopreservation. / Seo, S. A.; Yong, Taisoon; Im, K.

In: Kisaengch'unghak chapchi. The Korean journal of parasitology, Vol. 30, No. 2, 01.01.1992, p. 151-153.

Research output: Contribution to journalArticle

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