The medicinal plant Porana volubilis contains polysaccharides with anticoagulant activity mediated by heparin cofactor II

Seon Joo Yoon; Mariana S. Pereira; Mauro S.G. Pavão; Jae Kwan Hwang; Yu Ryang Pyun; Paulo A.S. Mourão

doi:10.1016/S0049-3848(02)00071-3

The medicinal plant Porana volubilis contains polysaccharides with anticoagulant activity mediated by heparin cofactor II

Seon Joo Yoon, Mariana S. Pereira, Mauro S.G. Pavão, Jae Kwan Hwang, Yu Ryang Pyun, Paulo A.S. Mourão

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47 Citations (Scopus)

Abstract

We searched for polysaccharides with anticoagulant activity and inhibitory action on platelet aggregation induced by collagen in 59 species of medicinal plants. We then concentrated our studies on the polysaccharide from the species Porana volubilis, which showed the highest anticoagulant activity among the plants tested. The polysaccharide from this species has an average molecular mass of ∼10 kDa, contains mainly galactose, galacturonic acid, and mannose but no sulfate esters. Its anticoagulant activity is mediated by the enhancement of thrombin inhibition that in turn is mediated by heparin cofactor II but not by antithrombin. The galacturonic acid residues are essential for activity since after reduction of its carboxyl groups the anticoagulant activity disappears. Our report is the first description of a natural nonsulfated polysaccharide from higher plants with anticoagulant activity, which may constitute a new source of compounds with action on coagulation and, perhaps, on thrombosis.

Original language	English
Pages (from-to)	51-58
Number of pages	8
Journal	Thrombosis Research
Volume	106
Issue number	1
DOIs	https://doi.org/10.1016/S0049-3848(02)00071-3
Publication status	Published - 2002 Apr 1

Bibliographical note

Funding Information:
This investigation was supported in part by grants from Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico (CNPq: FNDCT, PADCT, and PRONEX), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Financiadora de Estudos e Projetos (FINEP), and the Korea Science and Engineering Foundation (KOSEF) through the Bioproducts Research Center of Yonsei University. We are grateful to Adriana A.P. Correia for technical assistance.

All Science Journal Classification (ASJC) codes

Hematology

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abstract = "We searched for polysaccharides with anticoagulant activity and inhibitory action on platelet aggregation induced by collagen in 59 species of medicinal plants. We then concentrated our studies on the polysaccharide from the species Porana volubilis, which showed the highest anticoagulant activity among the plants tested. The polysaccharide from this species has an average molecular mass of ∼10 kDa, contains mainly galactose, galacturonic acid, and mannose but no sulfate esters. Its anticoagulant activity is mediated by the enhancement of thrombin inhibition that in turn is mediated by heparin cofactor II but not by antithrombin. The galacturonic acid residues are essential for activity since after reduction of its carboxyl groups the anticoagulant activity disappears. Our report is the first description of a natural nonsulfated polysaccharide from higher plants with anticoagulant activity, which may constitute a new source of compounds with action on coagulation and, perhaps, on thrombosis.",

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AU - Pyun, Yu Ryang

AU - Mourão, Paulo A.S.

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The medicinal plant Porana volubilis contains polysaccharides with anticoagulant activity mediated by heparin cofactor II

Abstract

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