The modulation of integrin expression by the extracellular matrix in articular chondrocytes

Sung Jae Kim, Eun Jung Kim, Yun Hee Kim, Soo Bong Hahn, Jin Woo Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Normal articular cartilage is composed chondrocytes embedded within an extracellular matrix (ECM). The patterns of integrin expression determine the adhesive properties of cells by modulating interactions with specific ECMS. Our hypothesis is that chondrocyte integrin expression changes in response to changes in their microenvironment. Porcine articular chondrocytes were encapsulated in alginate beads with several ECMs (collagen type I, collagen type II and fibronectin) for 7 days, subjected to RT-PCR, western blot analysis and immunofluorescence staining. It was found that chondrocytes in different ECMs showed different patterns of integrin expression. Integrin a5 and β1 were strongly expressed in all groups, but integrin a1 was strongly expressed only in collagen type I and fibronectin conjugated alginate beads, and integrin a2 was strongly expressed only in collagen type II conjugated alginate beads. These findings suggest that the addition of different ECMs to chondrocytes can modulate the patterns and levels of integrin expression possibly through a feedback mechanism. These finding suggest that the modulation of ECM interactions may play a critical role in the pathogenesis of osteoarthritis.

Original languageEnglish
Pages (from-to)493-501
Number of pages9
JournalYonsei medical journal
Volume44
Issue number3
DOIs
Publication statusPublished - 2003 Jun 30

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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