The modulation of neurotrophin and epigenetic regulators: Implication for astrocyte proliferation and neuronal cell apoptosis after spinal cord injury

Jong Heon Kim, Sung Hoon Kim, Sung Rae Cho, Ji Yong Lee, Ji Hyun Kim, Ahreum Baek, Hong Sun Jung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective To investigate alterations in the expression of the main regulators of neuronal survival and death related to astrocytes and neuronal cells in the brain in a mouse model of spinal cord injury (SCI). Methods Eight-week-old male imprinting control region mice (n=36; 30-35 g) were used in this study and randomly assigned to two groups: the naïve control group (n=18) and SCI group (n=18). The mice in both groups were randomly allocated to the following three time points: 3 days, 1 week, and 2 weeks (n=6 each). The expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), histone deacetylase 1 (HDAC1), and methyl-CpG-binding protein 2 (MeCP 2) in the brain were evaluated following thoracic contusive SCI. In addition, the number of neuronal cells in the motor cortex (M1 and M2 areas) and the number of astrocytes in the hippocampus were determined by immunohistochemistry. Results BDNF expression was significantly elevated at 2 weeks after injury (p=0.024). The GDNF level was significantly elevated at 3 days (p=0.042). The expression of HDAC1 was significantly elevated at 1 week (p=0.026). Following SCI, compared with the control the number of NeuN-positive cells in the M1 and M2 areas gradually and consistently decreased at 2 weeks after injury. In contrast, the number of astrocytes was significantly increased at 1 week (p=0.029). Conclusion These results demonstrate that the upregulation of BDNF, GDNF and HDAC1 might play on important role in brain reorganization after SCI.

Original languageEnglish
Pages (from-to)559-567
Number of pages9
JournalAnnals of Rehabilitation Medicine
Volume40
Issue number4
DOIs
Publication statusPublished - 2016 Jan 1

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Nerve Growth Factors
Spinal Cord Injuries
Epigenomics
Astrocytes
Histone Deacetylase 1
Glial Cell Line-Derived Neurotrophic Factor
Cell Proliferation
Apoptosis
Brain-Derived Neurotrophic Factor
Brain
Methyl-CpG-Binding Protein 2
Wounds and Injuries
Motor Cortex
Nerve Growth Factor
Hippocampus
Up-Regulation
Thorax
Cell Count
Immunohistochemistry
Control Groups

All Science Journal Classification (ASJC) codes

  • Rehabilitation

Cite this

Kim, Jong Heon ; Kim, Sung Hoon ; Cho, Sung Rae ; Lee, Ji Yong ; Kim, Ji Hyun ; Baek, Ahreum ; Jung, Hong Sun. / The modulation of neurotrophin and epigenetic regulators : Implication for astrocyte proliferation and neuronal cell apoptosis after spinal cord injury. In: Annals of Rehabilitation Medicine. 2016 ; Vol. 40, No. 4. pp. 559-567.
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abstract = "Objective To investigate alterations in the expression of the main regulators of neuronal survival and death related to astrocytes and neuronal cells in the brain in a mouse model of spinal cord injury (SCI). Methods Eight-week-old male imprinting control region mice (n=36; 30-35 g) were used in this study and randomly assigned to two groups: the na{\"i}ve control group (n=18) and SCI group (n=18). The mice in both groups were randomly allocated to the following three time points: 3 days, 1 week, and 2 weeks (n=6 each). The expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), histone deacetylase 1 (HDAC1), and methyl-CpG-binding protein 2 (MeCP 2) in the brain were evaluated following thoracic contusive SCI. In addition, the number of neuronal cells in the motor cortex (M1 and M2 areas) and the number of astrocytes in the hippocampus were determined by immunohistochemistry. Results BDNF expression was significantly elevated at 2 weeks after injury (p=0.024). The GDNF level was significantly elevated at 3 days (p=0.042). The expression of HDAC1 was significantly elevated at 1 week (p=0.026). Following SCI, compared with the control the number of NeuN-positive cells in the M1 and M2 areas gradually and consistently decreased at 2 weeks after injury. In contrast, the number of astrocytes was significantly increased at 1 week (p=0.029). Conclusion These results demonstrate that the upregulation of BDNF, GDNF and HDAC1 might play on important role in brain reorganization after SCI.",
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The modulation of neurotrophin and epigenetic regulators : Implication for astrocyte proliferation and neuronal cell apoptosis after spinal cord injury. / Kim, Jong Heon; Kim, Sung Hoon; Cho, Sung Rae; Lee, Ji Yong; Kim, Ji Hyun; Baek, Ahreum; Jung, Hong Sun.

In: Annals of Rehabilitation Medicine, Vol. 40, No. 4, 01.01.2016, p. 559-567.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The modulation of neurotrophin and epigenetic regulators

T2 - Implication for astrocyte proliferation and neuronal cell apoptosis after spinal cord injury

AU - Kim, Jong Heon

AU - Kim, Sung Hoon

AU - Cho, Sung Rae

AU - Lee, Ji Yong

AU - Kim, Ji Hyun

AU - Baek, Ahreum

AU - Jung, Hong Sun

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Objective To investigate alterations in the expression of the main regulators of neuronal survival and death related to astrocytes and neuronal cells in the brain in a mouse model of spinal cord injury (SCI). Methods Eight-week-old male imprinting control region mice (n=36; 30-35 g) were used in this study and randomly assigned to two groups: the naïve control group (n=18) and SCI group (n=18). The mice in both groups were randomly allocated to the following three time points: 3 days, 1 week, and 2 weeks (n=6 each). The expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), histone deacetylase 1 (HDAC1), and methyl-CpG-binding protein 2 (MeCP 2) in the brain were evaluated following thoracic contusive SCI. In addition, the number of neuronal cells in the motor cortex (M1 and M2 areas) and the number of astrocytes in the hippocampus were determined by immunohistochemistry. Results BDNF expression was significantly elevated at 2 weeks after injury (p=0.024). The GDNF level was significantly elevated at 3 days (p=0.042). The expression of HDAC1 was significantly elevated at 1 week (p=0.026). Following SCI, compared with the control the number of NeuN-positive cells in the M1 and M2 areas gradually and consistently decreased at 2 weeks after injury. In contrast, the number of astrocytes was significantly increased at 1 week (p=0.029). Conclusion These results demonstrate that the upregulation of BDNF, GDNF and HDAC1 might play on important role in brain reorganization after SCI.

AB - Objective To investigate alterations in the expression of the main regulators of neuronal survival and death related to astrocytes and neuronal cells in the brain in a mouse model of spinal cord injury (SCI). Methods Eight-week-old male imprinting control region mice (n=36; 30-35 g) were used in this study and randomly assigned to two groups: the naïve control group (n=18) and SCI group (n=18). The mice in both groups were randomly allocated to the following three time points: 3 days, 1 week, and 2 weeks (n=6 each). The expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), histone deacetylase 1 (HDAC1), and methyl-CpG-binding protein 2 (MeCP 2) in the brain were evaluated following thoracic contusive SCI. In addition, the number of neuronal cells in the motor cortex (M1 and M2 areas) and the number of astrocytes in the hippocampus were determined by immunohistochemistry. Results BDNF expression was significantly elevated at 2 weeks after injury (p=0.024). The GDNF level was significantly elevated at 3 days (p=0.042). The expression of HDAC1 was significantly elevated at 1 week (p=0.026). Following SCI, compared with the control the number of NeuN-positive cells in the M1 and M2 areas gradually and consistently decreased at 2 weeks after injury. In contrast, the number of astrocytes was significantly increased at 1 week (p=0.029). Conclusion These results demonstrate that the upregulation of BDNF, GDNF and HDAC1 might play on important role in brain reorganization after SCI.

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