The mutant leucine-zipper domain impairs both dimerization and suppressive function of Foxp3 in T cells

Wook Jin Chae, Octavian Henegariu, Sang Kyou Lee, Alfred L.M. Bothwell

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Regulatory T cells that express the Foxp3 transcription factor play important roles in preventing autoimmune diseases. Although several studies have demonstrated that the lack of the forkhead DNA-binding domain of Foxp3 caused severe autoimmune disease in scurfy mutant mice, the other functional domains of Foxp3 are less well characterized. Here, we show that the deletion of glutamic acid (ΔE250) in the leucine-zipper domain of Foxp3 causes a loss of hyporesponsiveness when compared with wild-type Foxp3 upon antigenic stimulation. CD4 T cells that ectopically express the glutamic acid mutant show significant losses of suppressor activity both in vitro and in vivo. We also demonstrate that regulation of both Th1- and Th2-type cytokine secretion in CD4 T cells that express wild-type Foxp3 is significantly altered by the deletion of glutamic acid. Defects are also observed in the expression of adhesion molecules, such as L-selectin (CD62L) and CD103, suggesting an important role of glutamic acid in the migratory behavior of regulatory T cells. Finally, this mutation reduces transcriptional repressor activity and impairs the homodimerization of Foxp3. Taken together, our results provide insight into the mechanism that controls autoimmune diseases via the deletion of this single glutamic acid residue in the leucine-zipper domain of Foxp3.

Original languageEnglish
Pages (from-to)9631-9636
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number25
DOIs
Publication statusPublished - 2006 Jun 20

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'The mutant leucine-zipper domain impairs both dimerization and suppressive function of Foxp3 in T cells'. Together they form a unique fingerprint.

  • Cite this