The mycobacterium avium subsp. paratuberculosis fibronectin attachment protein, a toll-like receptor 4 agonist, enhances dendritic cell-based cancer vaccine potency

Kyung Tae Noh, SungJae Shin, Kwang Hee Son, In Duk Jung, Hyun Kyu Kang, Su Jung Lee, Eun Kyung Lee, Yong Kyoo Shin, Ji Chang You, Yeong Min Park

Research output: Contribution to journalArticle

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Abstract

In this study, we showed the direct interaction between Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein (FAP) and toll-like receptor4 (TLR4) via co-localization and binding by using confocal microscopy and co-immunoprecipitation assays. FAP triggered the expression of pro- and antiinflammatory cytokines in a TLR4-dependent manner. In addition, FAP-induced cytokine expression in bone marrow-derived dendritic cells (BMDCs) was modulated in part by glycogen synthase kinase-3 (GSK-3). FAP-induced expression of CD80, CD86, major histocompatibility complex (MHC) class I, and MHC class II in TLR4 +/+ BMDCs was not observed in TLR4 -/- BMDCs. Furthermore, FAP induced DC-mediated CD8 + T cell proliferation and cytotoxic T lymphocyte (CTL) activity, and suppressed tumor growth with DC-based tumor vaccination in EG7 thymoma murine model. Taken together, these results indicate that the TLR4 agonist, FAP, a potential immunoadjuvant for DC-based cancer vaccination, improves the DC-based immune response via the TLR4 signaling pathway.

Original languageEnglish
Pages (from-to)340-349
Number of pages10
JournalExperimental and Molecular Medicine
Volume44
Issue number5
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Vaccine Potency
Paratuberculosis
Mycobacterium avium
Cancer Vaccines
Toll-Like Receptor 4
Fibronectins
Dendritic Cells
Bone
Proteins
T-cells
Bone Marrow
Major Histocompatibility Complex
Tumors
Vaccination
Cytokines
Glycogen Synthase Kinase 3
Immunologic Adjuvants
Neoplasms
Thymoma
Confocal microscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Noh, Kyung Tae ; Shin, SungJae ; Son, Kwang Hee ; Jung, In Duk ; Kang, Hyun Kyu ; Lee, Su Jung ; Lee, Eun Kyung ; Shin, Yong Kyoo ; You, Ji Chang ; Park, Yeong Min. / The mycobacterium avium subsp. paratuberculosis fibronectin attachment protein, a toll-like receptor 4 agonist, enhances dendritic cell-based cancer vaccine potency. In: Experimental and Molecular Medicine. 2012 ; Vol. 44, No. 5. pp. 340-349.
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The mycobacterium avium subsp. paratuberculosis fibronectin attachment protein, a toll-like receptor 4 agonist, enhances dendritic cell-based cancer vaccine potency. / Noh, Kyung Tae; Shin, SungJae; Son, Kwang Hee; Jung, In Duk; Kang, Hyun Kyu; Lee, Su Jung; Lee, Eun Kyung; Shin, Yong Kyoo; You, Ji Chang; Park, Yeong Min.

In: Experimental and Molecular Medicine, Vol. 44, No. 5, 01.01.2012, p. 340-349.

Research output: Contribution to journalArticle

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