The Mycobacterium avium subsp. Paratuberculosis protein MAP1305 modulates dendritic cell-mediated T cell proliferation through Toll-like receptor-4

Su Jung Lee, Kyung Tae Noh, Tae Heung Kang, Hee Dong Han, Sung Jae Shin, Byoung Yul Soh, Jung Hee Park, Yong Kyoo Shin, Han Wool Kim, Cheol Heui Yun, Won Sun Park, In Duk Jung, Yeong Min Park

Research output: Contribution to journalArticle

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Abstract

In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bone marrow-derived dendritic cells (BMDCs), a representative antigen presenting cell (APC). MAP1305 protein induces DC maturation and the production of pro-inflammatory cytokines (Interleukin (IL)-6), tumor necrosis factor (TNF)-α, and IL-1β) through Toll like receptor-4 (TLR-4) signaling by directly binding with TLR4. MAP1305 activates the phosphorylation of MAPKs, such as ERK, p38MAPK, and JNK, which is essential for DC maturation. Furthermore, MAP1305-treated DCs transform naive T cells to polarized CD4+ and CD8+ T cells, thus indicating a key role for this protein in the Th1 polarization of the resulting immune response. Taken together, M. avium subsp. paratuberculosis MAP1305 is important for the regulation of innate immune response through DC-mediated proliferation of CD4+ and CD8+ T cells.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalBMB reports
Volume47
Issue number2
DOIs
Publication statusPublished - 2014 Feb 1

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Paratuberculosis
Mycobacterium avium
Toll-Like Receptor 4
T-cells
Cell proliferation
Dendritic Cells
Cell Proliferation
T-Lymphocytes
Phosphorylation
Proteins
Antigen-Presenting Cells
Interleukin-1
Innate Immunity
Interleukin-6
Bone
Tumor Necrosis Factor-alpha
Bone Marrow
Polarization
Cytokines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Lee, Su Jung ; Noh, Kyung Tae ; Kang, Tae Heung ; Han, Hee Dong ; Shin, Sung Jae ; Soh, Byoung Yul ; Park, Jung Hee ; Shin, Yong Kyoo ; Kim, Han Wool ; Yun, Cheol Heui ; Park, Won Sun ; Jung, In Duk ; Park, Yeong Min. / The Mycobacterium avium subsp. Paratuberculosis protein MAP1305 modulates dendritic cell-mediated T cell proliferation through Toll-like receptor-4. In: BMB reports. 2014 ; Vol. 47, No. 2. pp. 115-120.
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abstract = "In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bone marrow-derived dendritic cells (BMDCs), a representative antigen presenting cell (APC). MAP1305 protein induces DC maturation and the production of pro-inflammatory cytokines (Interleukin (IL)-6), tumor necrosis factor (TNF)-α, and IL-1β) through Toll like receptor-4 (TLR-4) signaling by directly binding with TLR4. MAP1305 activates the phosphorylation of MAPKs, such as ERK, p38MAPK, and JNK, which is essential for DC maturation. Furthermore, MAP1305-treated DCs transform naive T cells to polarized CD4+ and CD8+ T cells, thus indicating a key role for this protein in the Th1 polarization of the resulting immune response. Taken together, M. avium subsp. paratuberculosis MAP1305 is important for the regulation of innate immune response through DC-mediated proliferation of CD4+ and CD8+ T cells.",
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Lee, SJ, Noh, KT, Kang, TH, Han, HD, Shin, SJ, Soh, BY, Park, JH, Shin, YK, Kim, HW, Yun, CH, Park, WS, Jung, ID & Park, YM 2014, 'The Mycobacterium avium subsp. Paratuberculosis protein MAP1305 modulates dendritic cell-mediated T cell proliferation through Toll-like receptor-4', BMB reports, vol. 47, no. 2, pp. 115-120. https://doi.org/10.5483/BMBRep.2014.47.2.277

The Mycobacterium avium subsp. Paratuberculosis protein MAP1305 modulates dendritic cell-mediated T cell proliferation through Toll-like receptor-4. / Lee, Su Jung; Noh, Kyung Tae; Kang, Tae Heung; Han, Hee Dong; Shin, Sung Jae; Soh, Byoung Yul; Park, Jung Hee; Shin, Yong Kyoo; Kim, Han Wool; Yun, Cheol Heui; Park, Won Sun; Jung, In Duk; Park, Yeong Min.

In: BMB reports, Vol. 47, No. 2, 01.02.2014, p. 115-120.

Research output: Contribution to journalArticle

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T1 - The Mycobacterium avium subsp. Paratuberculosis protein MAP1305 modulates dendritic cell-mediated T cell proliferation through Toll-like receptor-4

AU - Lee, Su Jung

AU - Noh, Kyung Tae

AU - Kang, Tae Heung

AU - Han, Hee Dong

AU - Shin, Sung Jae

AU - Soh, Byoung Yul

AU - Park, Jung Hee

AU - Shin, Yong Kyoo

AU - Kim, Han Wool

AU - Yun, Cheol Heui

AU - Park, Won Sun

AU - Jung, In Duk

AU - Park, Yeong Min

PY - 2014/2/1

Y1 - 2014/2/1

N2 - In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bone marrow-derived dendritic cells (BMDCs), a representative antigen presenting cell (APC). MAP1305 protein induces DC maturation and the production of pro-inflammatory cytokines (Interleukin (IL)-6), tumor necrosis factor (TNF)-α, and IL-1β) through Toll like receptor-4 (TLR-4) signaling by directly binding with TLR4. MAP1305 activates the phosphorylation of MAPKs, such as ERK, p38MAPK, and JNK, which is essential for DC maturation. Furthermore, MAP1305-treated DCs transform naive T cells to polarized CD4+ and CD8+ T cells, thus indicating a key role for this protein in the Th1 polarization of the resulting immune response. Taken together, M. avium subsp. paratuberculosis MAP1305 is important for the regulation of innate immune response through DC-mediated proliferation of CD4+ and CD8+ T cells.

AB - In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bone marrow-derived dendritic cells (BMDCs), a representative antigen presenting cell (APC). MAP1305 protein induces DC maturation and the production of pro-inflammatory cytokines (Interleukin (IL)-6), tumor necrosis factor (TNF)-α, and IL-1β) through Toll like receptor-4 (TLR-4) signaling by directly binding with TLR4. MAP1305 activates the phosphorylation of MAPKs, such as ERK, p38MAPK, and JNK, which is essential for DC maturation. Furthermore, MAP1305-treated DCs transform naive T cells to polarized CD4+ and CD8+ T cells, thus indicating a key role for this protein in the Th1 polarization of the resulting immune response. Taken together, M. avium subsp. paratuberculosis MAP1305 is important for the regulation of innate immune response through DC-mediated proliferation of CD4+ and CD8+ T cells.

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