Abstract
BACKGROUND: Mucoepidermoid carcinomas (MECs) are the most common malignant tumor of the salivary glands; however, the histogenesis of MECs has been still controversial. This study was undertaken to investigate the histogenesis of MECs by the examination of their collagen gel-based coculture tissue and transplanted tumors. METHODS: Two cell lines from a primary and a metastatic MECs were established and characterized by the mutational analysis of the p53 gene and in vivo tumorigenicity in athymic nude mice. Collagen gel-based organotypic cocultures were performed, and the ultrastructural and immuncohistochemical findings were examined. RESULTS: Two cell lines demonstrated p53 point mutation at the same codon. A metastatic cell line of MEC showed in vivo tumorigenicity. Transplanted tumors and the collagen gel-based culture tissues showed poorly differentiated squamous cell carcinomas devoid of mucous cell differentiation; however, they disclosed the differentiation of myoepithelial cells. CONCLUSIONS: MECs appear to be centered on the squamous cell differentiation, and the specific differentiation of myoepithelial or mucous cells seems to be modulated by the property of microenvironment.
| Original language | English |
|---|---|
| Pages (from-to) | 237-242 |
| Number of pages | 6 |
| Journal | Journal of Oral Pathology and Medicine |
| Volume | 33 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2004 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Oral Surgery
- Otorhinolaryngology
- Cancer Research
- Periodontics
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}
The myoepithelial cell differentiation of mucoepidermoid carcinoma in a collagen gel-based coculture model. / Yook, Jong In; Lee, Seoung Ae; Chun, Yong Chan; Huh, Jung; Cha, In Ho; Kim, Jin.
In: Journal of Oral Pathology and Medicine, Vol. 33, No. 4, 01.04.2004, p. 237-242.Research output: Contribution to journal › Article
TY - JOUR
T1 - The myoepithelial cell differentiation of mucoepidermoid carcinoma in a collagen gel-based coculture model
AU - Yook, Jong In
AU - Lee, Seoung Ae
AU - Chun, Yong Chan
AU - Huh, Jung
AU - Cha, In Ho
AU - Kim, Jin
PY - 2004/4/1
Y1 - 2004/4/1
N2 - BACKGROUND: Mucoepidermoid carcinomas (MECs) are the most common malignant tumor of the salivary glands; however, the histogenesis of MECs has been still controversial. This study was undertaken to investigate the histogenesis of MECs by the examination of their collagen gel-based coculture tissue and transplanted tumors. METHODS: Two cell lines from a primary and a metastatic MECs were established and characterized by the mutational analysis of the p53 gene and in vivo tumorigenicity in athymic nude mice. Collagen gel-based organotypic cocultures were performed, and the ultrastructural and immuncohistochemical findings were examined. RESULTS: Two cell lines demonstrated p53 point mutation at the same codon. A metastatic cell line of MEC showed in vivo tumorigenicity. Transplanted tumors and the collagen gel-based culture tissues showed poorly differentiated squamous cell carcinomas devoid of mucous cell differentiation; however, they disclosed the differentiation of myoepithelial cells. CONCLUSIONS: MECs appear to be centered on the squamous cell differentiation, and the specific differentiation of myoepithelial or mucous cells seems to be modulated by the property of microenvironment.
AB - BACKGROUND: Mucoepidermoid carcinomas (MECs) are the most common malignant tumor of the salivary glands; however, the histogenesis of MECs has been still controversial. This study was undertaken to investigate the histogenesis of MECs by the examination of their collagen gel-based coculture tissue and transplanted tumors. METHODS: Two cell lines from a primary and a metastatic MECs were established and characterized by the mutational analysis of the p53 gene and in vivo tumorigenicity in athymic nude mice. Collagen gel-based organotypic cocultures were performed, and the ultrastructural and immuncohistochemical findings were examined. RESULTS: Two cell lines demonstrated p53 point mutation at the same codon. A metastatic cell line of MEC showed in vivo tumorigenicity. Transplanted tumors and the collagen gel-based culture tissues showed poorly differentiated squamous cell carcinomas devoid of mucous cell differentiation; however, they disclosed the differentiation of myoepithelial cells. CONCLUSIONS: MECs appear to be centered on the squamous cell differentiation, and the specific differentiation of myoepithelial or mucous cells seems to be modulated by the property of microenvironment.
UR - http://www.scopus.com/inward/record.url?scp=1842734918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842734918&partnerID=8YFLogxK
U2 - 10.1111/j.0904-2512.2004.00056.x
DO - 10.1111/j.0904-2512.2004.00056.x
M3 - Article
C2 - 15061712
AN - SCOPUS:1842734918
VL - 33
SP - 237
EP - 242
JO - Journal of Oral Pathology and Medicine
JF - Journal of Oral Pathology and Medicine
SN - 0904-2512
IS - 4
ER -