The novel function of Oct3/4 in mouse tooth development

Eizo Nakagawa, Li Zhang, Eun Jung Kim, Jeong Oh Shin, Sung Won Cho, Hayato Ohshima, Han Sung Jung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Octamer-binding factor 3/4 (Oct3/4) is one of the key regulators maintaining the pluripotency and self-renewal in embryonic stem cells and is involved in the developmental events. However, the functional significance of Oct3/4 remains to be clarified during tooth morphogenesis. This study aimed to examine the functional role of Oct3/4 in mouse. During tooth morphogenesis (E11-E16.5), Oct3/4-positive cells, detected by nuclear immunoreaction, increased in number, and subsequently, their immunoreaction shifted from the nucleus to the cytoplasm at the stage of cell differentiation (E18.5). Quantitative real-time PCR clearly demonstrated the relationship between isoforms of Oct3/4 and the in vivo cellular localization of Oct3/4, suggesting that the Oct3/4 expressed in nucleus was Oct3/4A, whereas that expressed in the cytoplasm was Oct3/4B. RNAi knockdown of Oct3/4 induced apoptosis and arrested tooth morphogenesis. Our results suggest that (1) the increased number of Oct3/4-positive cells with nuclear immunoreaction correlate with active cell proliferation during tooth morphogenesis and (2) the shift of Oct3/4 from the nucleus to the cytoplasm plays a crucial role in cell differentiation.

Original languageEnglish
Pages (from-to)367-376
Number of pages10
JournalHistochemistry and cell biology
Volume137
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

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Morphogenesis
Tooth
Cytoplasm
Cell Differentiation
Embryonic Stem Cells
RNA Interference
Real-Time Polymerase Chain Reaction
Protein Isoforms
Cell Proliferation
Apoptosis

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Nakagawa, E., Zhang, L., Kim, E. J., Shin, J. O., Cho, S. W., Ohshima, H., & Jung, H. S. (2012). The novel function of Oct3/4 in mouse tooth development. Histochemistry and cell biology, 137(3), 367-376. https://doi.org/10.1007/s00418-011-0895-y
Nakagawa, Eizo ; Zhang, Li ; Kim, Eun Jung ; Shin, Jeong Oh ; Cho, Sung Won ; Ohshima, Hayato ; Jung, Han Sung. / The novel function of Oct3/4 in mouse tooth development. In: Histochemistry and cell biology. 2012 ; Vol. 137, No. 3. pp. 367-376.
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Nakagawa, E, Zhang, L, Kim, EJ, Shin, JO, Cho, SW, Ohshima, H & Jung, HS 2012, 'The novel function of Oct3/4 in mouse tooth development', Histochemistry and cell biology, vol. 137, no. 3, pp. 367-376. https://doi.org/10.1007/s00418-011-0895-y

The novel function of Oct3/4 in mouse tooth development. / Nakagawa, Eizo; Zhang, Li; Kim, Eun Jung; Shin, Jeong Oh; Cho, Sung Won; Ohshima, Hayato; Jung, Han Sung.

In: Histochemistry and cell biology, Vol. 137, No. 3, 01.03.2012, p. 367-376.

Research output: Contribution to journalArticle

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Nakagawa E, Zhang L, Kim EJ, Shin JO, Cho SW, Ohshima H et al. The novel function of Oct3/4 in mouse tooth development. Histochemistry and cell biology. 2012 Mar 1;137(3):367-376. https://doi.org/10.1007/s00418-011-0895-y