The novel function of Oct3/4 in mouse tooth development

Eizo Nakagawa, Li Zhang, Eun Jung Kim, Jeong Oh Shin, Sung Won Cho, Hayato Ohshima, Han Sung Jung

Research output: Contribution to journalArticle

13 Citations (Scopus)


Octamer-binding factor 3/4 (Oct3/4) is one of the key regulators maintaining the pluripotency and self-renewal in embryonic stem cells and is involved in the developmental events. However, the functional significance of Oct3/4 remains to be clarified during tooth morphogenesis. This study aimed to examine the functional role of Oct3/4 in mouse. During tooth morphogenesis (E11-E16.5), Oct3/4-positive cells, detected by nuclear immunoreaction, increased in number, and subsequently, their immunoreaction shifted from the nucleus to the cytoplasm at the stage of cell differentiation (E18.5). Quantitative real-time PCR clearly demonstrated the relationship between isoforms of Oct3/4 and the in vivo cellular localization of Oct3/4, suggesting that the Oct3/4 expressed in nucleus was Oct3/4A, whereas that expressed in the cytoplasm was Oct3/4B. RNAi knockdown of Oct3/4 induced apoptosis and arrested tooth morphogenesis. Our results suggest that (1) the increased number of Oct3/4-positive cells with nuclear immunoreaction correlate with active cell proliferation during tooth morphogenesis and (2) the shift of Oct3/4 from the nucleus to the cytoplasm plays a crucial role in cell differentiation.

Original languageEnglish
Pages (from-to)367-376
Number of pages10
JournalHistochemistry and cell biology
Issue number3
Publication statusPublished - 2012 Mar 1

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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  • Cite this

    Nakagawa, E., Zhang, L., Kim, E. J., Shin, J. O., Cho, S. W., Ohshima, H., & Jung, H. S. (2012). The novel function of Oct3/4 in mouse tooth development. Histochemistry and cell biology, 137(3), 367-376.