The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis

Young Jeon, Yong Joon Park, Hui Kyung Cho, Hyun Ju Jung, Tae Kyu Ahn, Hunseung Kang, Hyunsook Pai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Nucleostemin is a nucleolar GTP-binding protein that is involved in stem cell proliferation, embryonic development, and ribosome biogenesis in mammals. Plant nucleostemin-like 1 (NSN1) plays a role in embryogenesis, and apical and foral meristem development. In this study, a nucleolar function of NSN1 in the regulation of ribosome biogenesis was identifed. Green fuorescent protein (GFP)-fused NSN1 localized to the nucleolus, which was primarily determined by its N-terminal domain. Recombinant NSN1 and its N-terminal domain (NSN1-N) bound to RNA in vitro. Recombinant NSN1 expressed GTPase activity in vitro. NSN1 silencing in Arabidopsis thaliana and Nicotiana benthamiana led to growth retardation and premature senescence. NSN1 interacted with Pescadillo and EBNA1 binding protein 2 (EBP2), which are nucleolar proteins involved in ribosome biogenesis, and with several ribosomal proteins. NSN1, NSN1-N, and EBP2 co-fractionated primarily with the 60S ribosomal large subunit in vivo. Depletion of NSN1 delayed 25S rRNA maturation and biogenesis of the 60S ribosome subunit, and repressed global translation. NSN1-defcient plants exhibited premature leaf senescence, excessive accumulation of reactive oxygen species, and senescence-related gene expression. Taken together, these results suggest that NSN1 plays a crucial role in plant growth and senescence by modulating ribosome biogenesis.

Original languageEnglish
Pages (from-to)6297-6310
Number of pages14
JournalJournal of Experimental Botany
Volume66
Issue number20
DOIs
Publication statusPublished - 2015 Jan 1

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GTP Phosphohydrolases
guanosinetriphosphatase
ribosomes
Ribosomes
Eukaryotic Large Ribosome Subunits
plant growth
Growth
Nuclear Proteins
Embryonic Development
Carrier Proteins
binding proteins
embryogenesis
Meristem
Ribosomal Proteins
GTP-Binding Proteins
Arabidopsis
Nicotiana benthamiana
Tobacco
ribosomal proteins
cell nucleolus

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Jeon, Young ; Park, Yong Joon ; Cho, Hui Kyung ; Jung, Hyun Ju ; Ahn, Tae Kyu ; Kang, Hunseung ; Pai, Hyunsook. / The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis. In: Journal of Experimental Botany. 2015 ; Vol. 66, No. 20. pp. 6297-6310.
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The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis. / Jeon, Young; Park, Yong Joon; Cho, Hui Kyung; Jung, Hyun Ju; Ahn, Tae Kyu; Kang, Hunseung; Pai, Hyunsook.

In: Journal of Experimental Botany, Vol. 66, No. 20, 01.01.2015, p. 6297-6310.

Research output: Contribution to journalArticle

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