The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish

Hyouk Bum Kwon, Shigetomo Fukuhara, Kazuhide Asakawa, Koji Ando, Takeru Kashiwada, Koichi Kawakami, Masahiko Hibi, Young-Guen Kwon, Kyu Won Kim, Kari Alitalo, Naoki Mochizuki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Blood vessels and neurons grow often side by side. However, the molecular and cellular mechanisms underlying their parallel development remain unclear. Here, we report that a subpopulation of secondary motoneurons extends axons ventrally outside of the neural tubes and rostrocaudally as a fascicle beneath the dorsal aorta (DA) in zebrafish. We tried to clarify the mechanism by which these motoneuron axons grow beneath the DA and found that Vegfc in the DA and Vegfr3 in the motoneurons were essential for the axon growth. Forced expression of either Vegfc in arteries or Vegfr3 in motoneurons resulted in enhanced axon growth of motoneurons over the DA. Both vegfr3 morphants and vegfc morphants lost the alignment of motoneuron axons with DA. In addition, forced expression of two mutant forms of Vegfr3 in motoneurons, potentially trapping endogenous Vegfc, resulted in failure of growth of motoneuron axons beneath the DA. Finally, a vegfr3 mutant fish lacked the motoneuron axons beneath the DA. Collectively, Vegfc from the preformed DA guides the axon growth of secondary motoneurons.

Original languageEnglish
Pages (from-to)4081-4090
Number of pages10
JournalDevelopment (Cambridge)
Volume140
Issue number19
DOIs
Publication statusPublished - 2013 Oct 1

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Motor Neurons
Zebrafish
Axons
Aorta
Growth
Neural Tube
Blood Vessels
Fishes
Arteries
Neurons

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Kwon, H. B., Fukuhara, S., Asakawa, K., Ando, K., Kashiwada, T., Kawakami, K., ... Mochizuki, N. (2013). The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish. Development (Cambridge), 140(19), 4081-4090. https://doi.org/10.1242/dev.091702
Kwon, Hyouk Bum ; Fukuhara, Shigetomo ; Asakawa, Kazuhide ; Ando, Koji ; Kashiwada, Takeru ; Kawakami, Koichi ; Hibi, Masahiko ; Kwon, Young-Guen ; Kim, Kyu Won ; Alitalo, Kari ; Mochizuki, Naoki. / The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish. In: Development (Cambridge). 2013 ; Vol. 140, No. 19. pp. 4081-4090.
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abstract = "Blood vessels and neurons grow often side by side. However, the molecular and cellular mechanisms underlying their parallel development remain unclear. Here, we report that a subpopulation of secondary motoneurons extends axons ventrally outside of the neural tubes and rostrocaudally as a fascicle beneath the dorsal aorta (DA) in zebrafish. We tried to clarify the mechanism by which these motoneuron axons grow beneath the DA and found that Vegfc in the DA and Vegfr3 in the motoneurons were essential for the axon growth. Forced expression of either Vegfc in arteries or Vegfr3 in motoneurons resulted in enhanced axon growth of motoneurons over the DA. Both vegfr3 morphants and vegfc morphants lost the alignment of motoneuron axons with DA. In addition, forced expression of two mutant forms of Vegfr3 in motoneurons, potentially trapping endogenous Vegfc, resulted in failure of growth of motoneuron axons beneath the DA. Finally, a vegfr3 mutant fish lacked the motoneuron axons beneath the DA. Collectively, Vegfc from the preformed DA guides the axon growth of secondary motoneurons.",
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Kwon, HB, Fukuhara, S, Asakawa, K, Ando, K, Kashiwada, T, Kawakami, K, Hibi, M, Kwon, Y-G, Kim, KW, Alitalo, K & Mochizuki, N 2013, 'The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish', Development (Cambridge), vol. 140, no. 19, pp. 4081-4090. https://doi.org/10.1242/dev.091702

The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish. / Kwon, Hyouk Bum; Fukuhara, Shigetomo; Asakawa, Kazuhide; Ando, Koji; Kashiwada, Takeru; Kawakami, Koichi; Hibi, Masahiko; Kwon, Young-Guen; Kim, Kyu Won; Alitalo, Kari; Mochizuki, Naoki.

In: Development (Cambridge), Vol. 140, No. 19, 01.10.2013, p. 4081-4090.

Research output: Contribution to journalArticle

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AU - Kwon, Hyouk Bum

AU - Fukuhara, Shigetomo

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AU - Ando, Koji

AU - Kashiwada, Takeru

AU - Kawakami, Koichi

AU - Hibi, Masahiko

AU - Kwon, Young-Guen

AU - Kim, Kyu Won

AU - Alitalo, Kari

AU - Mochizuki, Naoki

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N2 - Blood vessels and neurons grow often side by side. However, the molecular and cellular mechanisms underlying their parallel development remain unclear. Here, we report that a subpopulation of secondary motoneurons extends axons ventrally outside of the neural tubes and rostrocaudally as a fascicle beneath the dorsal aorta (DA) in zebrafish. We tried to clarify the mechanism by which these motoneuron axons grow beneath the DA and found that Vegfc in the DA and Vegfr3 in the motoneurons were essential for the axon growth. Forced expression of either Vegfc in arteries or Vegfr3 in motoneurons resulted in enhanced axon growth of motoneurons over the DA. Both vegfr3 morphants and vegfc morphants lost the alignment of motoneuron axons with DA. In addition, forced expression of two mutant forms of Vegfr3 in motoneurons, potentially trapping endogenous Vegfc, resulted in failure of growth of motoneuron axons beneath the DA. Finally, a vegfr3 mutant fish lacked the motoneuron axons beneath the DA. Collectively, Vegfc from the preformed DA guides the axon growth of secondary motoneurons.

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