The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization.

Min Young Seo, SunYoung Rha, Sang Hwa Yang, Sang Cheol Kim, Gui Youn Lee, Chan Hee Park, Woo Ick Yang, Joong Bae Ahn, Byeongwoo Park, Hyuncheol Chung

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The nature of genetic alterations in bilateral breast cancer (BBC) associated with the distinctive development of a second primary tumor or a metastatic lesion is not clearly established. In this study, patterns of promoter methylation and gene copy number changes were assessed for their utility in the distinction of two types of BBC (synchronous and metachronous). Seven cases of synchronous and five cases of metachronous breast cancer tissues were used in X chromosome inactivation assay to assess the methylation pattern of human androgen receptor gene. X chromosome inactivation assay alone did not provide enough information to distinguish the genetic origins of synchronous and metachronous BBC. When four pairs of paraffin-embedded BBC tissues were used in cDNA array-based CGH with placenta DNA as a reference, higher DNA copy number changes were observed from metachronous pairs (9.0%) than from synchronous pairs (3.1%). From the two cases of metachronous pairs tested, 44 genes were found to be commonly modulated in gene copy numbers in a cancer detected later.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalInternational Journal of Molecular Medicine
Volume13
Issue number1
Publication statusPublished - 2004 Jan 1

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Comparative Genomic Hybridization
Gene Dosage
Oligonucleotide Array Sequence Analysis
Breast Neoplasms
X Chromosome Inactivation
Methylation
DNA Copy Number Variations
Paraffin
Placenta
Genes
Neoplasms
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Seo, Min Young ; Rha, SunYoung ; Yang, Sang Hwa ; Kim, Sang Cheol ; Lee, Gui Youn ; Park, Chan Hee ; Yang, Woo Ick ; Ahn, Joong Bae ; Park, Byeongwoo ; Chung, Hyuncheol. / The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization. In: International Journal of Molecular Medicine. 2004 ; Vol. 13, No. 1. pp. 17-24.
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The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization. / Seo, Min Young; Rha, SunYoung; Yang, Sang Hwa; Kim, Sang Cheol; Lee, Gui Youn; Park, Chan Hee; Yang, Woo Ick; Ahn, Joong Bae; Park, Byeongwoo; Chung, Hyuncheol.

In: International Journal of Molecular Medicine, Vol. 13, No. 1, 01.01.2004, p. 17-24.

Research output: Contribution to journalArticle

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