The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis.

So Young Yi, Jee Hyub Kim, Young Hee Joung, Sanghyeob Lee, Woo Taek Kim, Seung Hun Yu, Doil Choi

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels of CaPF1 transcripts were observed in disease-resistant tissue compared with susceptible tissue. CaPF1 expression was additionally induced using various treatment regimes, including ethephon, methyl jasmonate, and cold stress. To determine the role of CaPF1 in plants, transgenic Arabidopsis and tobacco (Nicotiana tabacum) plants expressing higher levels of CaPF1 were generated. Gene expression analyses of transgenic Arabidopsis and tobacco revealed that the CaPF1 level in transgenic plants affects expression of genes that contain either a GCC or a CRT/DRE box in their promoter regions. Furthermore, transgenic Arabidopsis plants expressing CaPF1 displayed tolerance against freezing temperatures and enhanced resistance to Pseudomonas syringae pv tomato DC3000. Disease tolerance was additionally observed in CaPF1 transgenic tobacco plants. The results collectively indicate that CaPF1 is an ERF/AP2 transcription factor in hot pepper plants that may play dual roles in response to biotic and abiotic stress in plants.

Original languageEnglish
Pages (from-to)2862-2874
Number of pages13
JournalPlant physiology
Volume136
Issue number1
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Genetically Modified Plants
sweet peppers
Arabidopsis
cold tolerance
Freezing
Tobacco
Transcription Factors
transcription factors
Transcription Factor AP-2
Capsicum
pathogens
tobacco
genetically modified organisms
Xanthomonas axonopodis
biotic stress
abiotic stress
transgenic plants
Pseudomonas syringae
Gene Expression
Lycopersicon esculentum

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Yi, So Young ; Kim, Jee Hyub ; Joung, Young Hee ; Lee, Sanghyeob ; Kim, Woo Taek ; Yu, Seung Hun ; Choi, Doil. / The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis. In: Plant physiology. 2004 ; Vol. 136, No. 1. pp. 2862-2874.
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abstract = "An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels of CaPF1 transcripts were observed in disease-resistant tissue compared with susceptible tissue. CaPF1 expression was additionally induced using various treatment regimes, including ethephon, methyl jasmonate, and cold stress. To determine the role of CaPF1 in plants, transgenic Arabidopsis and tobacco (Nicotiana tabacum) plants expressing higher levels of CaPF1 were generated. Gene expression analyses of transgenic Arabidopsis and tobacco revealed that the CaPF1 level in transgenic plants affects expression of genes that contain either a GCC or a CRT/DRE box in their promoter regions. Furthermore, transgenic Arabidopsis plants expressing CaPF1 displayed tolerance against freezing temperatures and enhanced resistance to Pseudomonas syringae pv tomato DC3000. Disease tolerance was additionally observed in CaPF1 transgenic tobacco plants. The results collectively indicate that CaPF1 is an ERF/AP2 transcription factor in hot pepper plants that may play dual roles in response to biotic and abiotic stress in plants.",
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The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis. / Yi, So Young; Kim, Jee Hyub; Joung, Young Hee; Lee, Sanghyeob; Kim, Woo Taek; Yu, Seung Hun; Choi, Doil.

In: Plant physiology, Vol. 136, No. 1, 01.01.2004, p. 2862-2874.

Research output: Contribution to journalArticle

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