The PI3 kinase-Akt pathway mediates Wnt3a-induced proliferation

Sung Eun Kim, Won Jeong Lee, Kang-Yell Choi

Research output: Contribution to journalArticle

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Abstract

Wnt3a activates proliferation of fibroblasts cells via activation of both extracellular signal-regulated kinase (ERK) and Wnt/β-catenin signaling pathways. In this study, we show that the phosphatidyl inositol 3 kinases (PI3K)-Akt pathway is also involved in the Wnt3a-induced proliferation. Akt was activated within 30 min by Wnt3a in NIH3T3 cells. By Wnt3a treatment, activated Akt was transiently accumulated in nucleus although β-catenin was accumulated in the nucleus of cells in a prolonged manner. The Wnt3a-induced Akt activation was not affected by siRNA-mediated reduction of β-catenin, indicating that Wnt3a-induced Akt activation may occur independently of β-catenin. The Wnt3a-induced Akt activation was abolished by pre-treatment with PI3K inhibitor, LY294002 and Wortmanin, but not by MEK inhibitor, U0126, indicating that Wnt3a activates Akt via PI3K. The growth and proliferation induced by Wnt3a were blocked by treatments of the PI3K inhibitors. Furthermore, Wnt3a-induced proliferation was blocked by Akt siRNA. These results reveal that the PI3K-Akt pathway mediates the Wnt3a-induced growth and proliferation of NIH3T3 cells.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalCellular Signalling
Volume19
Issue number3
DOIs
Publication statusPublished - 2007 Mar 1

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Phosphatidylinositols
Catenins
Phosphatidylinositol 3-Kinases
Phosphotransferases
Small Interfering RNA
Cell Proliferation
Wnt Signaling Pathway
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Growth
Cell Nucleus
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Kim, Sung Eun ; Lee, Won Jeong ; Choi, Kang-Yell. / The PI3 kinase-Akt pathway mediates Wnt3a-induced proliferation. In: Cellular Signalling. 2007 ; Vol. 19, No. 3. pp. 511-518.
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The PI3 kinase-Akt pathway mediates Wnt3a-induced proliferation. / Kim, Sung Eun; Lee, Won Jeong; Choi, Kang-Yell.

In: Cellular Signalling, Vol. 19, No. 3, 01.03.2007, p. 511-518.

Research output: Contribution to journalArticle

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