The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development

Ryan S. Gray, Philip B. Abitua, Bogdan J. Wlodarczyk, Heather L. Szabo-Rogers, Otis Blanchard, Insuk Lee, Greg S. Weiss, Karen J. Liu, Edward M. Marcotte, John B. Wallingford, Richard H. Finnell

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The planar cell polarity (PCP) signalling pathway is essential for embryonic development because it governs diverse cellular behaviours, and 'core PCP' proteins, such as Dishevelled and Frizzled, have been extensively characterized. By contrast, the 'PCP effector' proteins, such as Intu and Fuz, remain largely unstudied. These proteins are essential for PCP signalling, but they have never been investigated in mammals and their cell biological activities remain entirely unknown. We report here that Fuz mutant mice show neural tube defects, skeletal dysmorphologies and Hedgehog signalling defects stemming from disrupted ciliogenesis. Using bioinformatics and imaging of an in vivo mucociliary epithelium, we established a central role for Fuz in membrane trafficking, showing that Fuz is essential for trafficking of cargo to basal bodies and to the apical tips of cilia. Fuz is also essential for exocytosis in secretory cells. Finally, we identified a Rab-related small GTPase as a Fuz interaction partner that is also essential for ciliogenesis and secretion. These results are significant because they provide new insights into the mechanisms by which developmental regulatory systems such as PCP signalling interface with fundamental cellular systems such as the vesicle trafficking machinery.

Original languageEnglish
Pages (from-to)1225-1232
Number of pages8
JournalNature Cell Biology
Volume11
Issue number10
DOIs
Publication statusPublished - 2009 Sep 21

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Cell Polarity
Embryonic Development
Membranes
Basal Bodies
Proteins
Monomeric GTP-Binding Proteins
Hedgehogs
Neural Tube Defects
Cilia
Exocytosis
Computational Biology
Mammals
Epithelium

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Gray, R. S., Abitua, P. B., Wlodarczyk, B. J., Szabo-Rogers, H. L., Blanchard, O., Lee, I., ... Finnell, R. H. (2009). The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development. Nature Cell Biology, 11(10), 1225-1232. https://doi.org/10.1038/ncb1966
Gray, Ryan S. ; Abitua, Philip B. ; Wlodarczyk, Bogdan J. ; Szabo-Rogers, Heather L. ; Blanchard, Otis ; Lee, Insuk ; Weiss, Greg S. ; Liu, Karen J. ; Marcotte, Edward M. ; Wallingford, John B. ; Finnell, Richard H. / The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development. In: Nature Cell Biology. 2009 ; Vol. 11, No. 10. pp. 1225-1232.
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Gray, RS, Abitua, PB, Wlodarczyk, BJ, Szabo-Rogers, HL, Blanchard, O, Lee, I, Weiss, GS, Liu, KJ, Marcotte, EM, Wallingford, JB & Finnell, RH 2009, 'The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development', Nature Cell Biology, vol. 11, no. 10, pp. 1225-1232. https://doi.org/10.1038/ncb1966

The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development. / Gray, Ryan S.; Abitua, Philip B.; Wlodarczyk, Bogdan J.; Szabo-Rogers, Heather L.; Blanchard, Otis; Lee, Insuk; Weiss, Greg S.; Liu, Karen J.; Marcotte, Edward M.; Wallingford, John B.; Finnell, Richard H.

In: Nature Cell Biology, Vol. 11, No. 10, 21.09.2009, p. 1225-1232.

Research output: Contribution to journalArticle

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