The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a

Se Yeon Lee, Onju Ham, Min Ji Cha, Byeong Wook Song, Eunmi Choi, Il Kwon Kim, Woochul Chang, Soyeon Lim, Chang Youn Lee, Jun Hee Park, Jiyun Lee, Yoonjin Bae, Hyang Hee Seo, Eunhyun Choi, Yangsoo Jang, Ki Chul Hwang

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Abstract

Human bone marrow-derived mesenchymal stem cells (hMSCs) are an attractive candidate for cell therapy in heart disease. Low survival and incomplete electromechanical integration between resident cardiomyocytes and transplanted hMSCs remain unsolved. In order for an infarcted heart to tolerate transplantation, differentiation capacity in stem cells must be reinforced. In this study, we found that compound 56, an epidermal growth factor receptor (EGFR) inhibitor, promotes cardiogenic differentiation of hMSCs and the transplantation of hMSCs treated with compound 56 resulted in enhancement of heart functions. Furthermore, hMSCs transfected with microRNA-133a (miR-133a), which targets EGFR, were observed to express cardiac-specific markers. We also discovered that luciferase activity is exclusively decreased by targeting EGFR in hMSCs transfected with miR-133a mimic. These results suggest that EGFR plays a key role in the regulation of cardiogenic differentiation in hMSCs.

Original languageEnglish
Pages (from-to)92-99
Number of pages8
JournalBiomaterials
Volume34
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Lee, S. Y., Ham, O., Cha, M. J., Song, B. W., Choi, E., Kim, I. K., Chang, W., Lim, S., Lee, C. Y., Park, J. H., Lee, J., Bae, Y., Seo, H. H., Choi, E., Jang, Y., & Hwang, K. C. (2013). The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a. Biomaterials, 34(1), 92-99. https://doi.org/10.1016/j.biomaterials.2012.09.069