The promotion of human neural stem cells adhesion using bioinspired poly(norepinephrine) nanoscale coating

Minah Park, Mikyung Shin, Eunmi Kim, Slgirim Lee, Kook In Park, Haeshin Lee, Jae-Hyung Jang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The establishment of versatile biomaterial interfaces that can facilitate cellular adhesion is crucial for elucidating the cellular processes that occur on biomaterial surfaces. Furthermore, biomaterial interfaces can provide physical or chemical cues that are capable of stimulating cellular behaviors by regulating intracellular signaling cascades. Herein, a method of creating a biomimetic functional biointerface was introduced to enhance human neural stem cell (hNSC) adhesion. The hNSC-compatible biointerface was prepared by the oxidative polymerization of the neurotransmitter norepinephrine, which generates a nanoscale organic thin layer, termed poly(norepinephrine) (pNE). Due to its adhesive property, pNE resulted in an adherent layer on various substrates, and pNE-coated biointerfaces provided a highly favorable microenvironment for hNSCs, with no observed cytotoxicity. Only a 2-hour incubation of hNSCs was required to firmly attach the stem cells, regardless of the type of substrate. Importantly, the adhesive properties of pNE interfaces led to micropatterns of cellular attachment, thereby demonstrating the ability of the interface to organize the stem cells. This highly facile surface-modification method using a biomimetic pNE thin layer can be applied to a number of suitable materials that were previously not compatible with hNSC technology.

Original languageEnglish
Article number793052
JournalJournal of Nanomaterials
Volume2014
DOIs
Publication statusPublished - 2014 Jan 1

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Norepinephrine
Cell adhesion
Stem cells
Coatings
Biocompatible Materials
Biomaterials
Biomimetics
Adhesives
Substrates
Cytotoxicity
Neurotransmitter Agents
Surface treatment
Adhesion
Polymerization

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Park, Minah ; Shin, Mikyung ; Kim, Eunmi ; Lee, Slgirim ; Park, Kook In ; Lee, Haeshin ; Jang, Jae-Hyung. / The promotion of human neural stem cells adhesion using bioinspired poly(norepinephrine) nanoscale coating. In: Journal of Nanomaterials. 2014 ; Vol. 2014.
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The promotion of human neural stem cells adhesion using bioinspired poly(norepinephrine) nanoscale coating. / Park, Minah; Shin, Mikyung; Kim, Eunmi; Lee, Slgirim; Park, Kook In; Lee, Haeshin; Jang, Jae-Hyung.

In: Journal of Nanomaterials, Vol. 2014, 793052, 01.01.2014.

Research output: Contribution to journalArticle

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