The protective effect of melatonin on neural stem cell against LPS-induced inflammation

Juhyun Song, So Mang Kang, Kyoung Min Lee, Jong Eun Lee

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Stem cell therapy for tissue regeneration has several limitations in the fact that transplanted cells could not survive for a long time. For solving these limitations, many studies have focused on the antioxidants to increase survival rate of neural stem cells (NSCs). Melatonin, an antioxidant synthesized in the pineal gland, plays multiple roles in various physiological mechanisms. Melatonin exerts neuroprotective effects in the central nervous system. To determine the effect of melatonin on NSCs which is in LPS-induced inflammatory stress state, we first investigated nitric oxide (NO) production and cytotoxicity using Griess reagent assays, LDH assay, and neurosphere counting. Also, we investigated the effect of melatonin on NSCs by measuring the mRNA levels of SOX2, TLX, and FGFR-2. In addition, western blot analyses were performed to examine the activation of PI3K/Akt/Nrf2 signaling in LPS-treated NSCs. In the present study, we suggested that melatonin inhibits NO production and protects NSCs against LPS-induced inflammatory stress. In addition, melatonin promoted the expression of SOX2 and activated the PI3K/Akt/Nrf2 signaling under LPS-induced inflammation condition. Based on our results, we conclude that melatonin may be an important factor for the survival and proliferation of NSCs in neuroinflammatory diseases.

Original languageEnglish
Article number854359
JournalBioMed Research International
Volume2015
DOIs
Publication statusPublished - 2015

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Neural Stem Cells
Melatonin
Stem cells
Inflammation
Phosphatidylinositol 3-Kinases
Assays
Nitric Oxide
Antioxidants
Tissue regeneration
Pineal Gland
Neurology
Neuroprotective Agents
Cytotoxicity
Cell- and Tissue-Based Therapy
Regeneration
Stem Cells
Central Nervous System
Western Blotting
Chemical activation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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The protective effect of melatonin on neural stem cell against LPS-induced inflammation. / Song, Juhyun; Kang, So Mang; Lee, Kyoung Min; Lee, Jong Eun.

In: BioMed Research International, Vol. 2015, 854359, 2015.

Research output: Contribution to journalArticle

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