The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants

Sang Gon Kim, Sun Tae Kim, Yiming Wang, Seok Yu, In Soo Choi, Yong Chul Kim, Woo Taek Kim, Ganesh Kumar Agrawal, Randeep Rakwal, Kyu Young Kang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumulate in tissues undergoing cell death. However, the function of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dosedependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the mechanism by which PBZ1 confers the cell death morphology in rice and other model plants.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalMolecules and cells
Volume31
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

Ribonucleases
Cell Death
Arabidopsis
Proteins
Dexamethasone
Tobacco
Cultured Cells
Suspensions
oryzemate
Oryza
In Situ Nick-End Labeling
DNA Fragmentation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Kim, S. G., Kim, S. T., Wang, Y., Yu, S., Choi, I. S., Kim, Y. C., ... Kang, K. Y. (2011). The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants. Molecules and cells, 31(1), 25-31. https://doi.org/10.1007/s10059-011-0004-z
Kim, Sang Gon ; Kim, Sun Tae ; Wang, Yiming ; Yu, Seok ; Choi, In Soo ; Kim, Yong Chul ; Kim, Woo Taek ; Agrawal, Ganesh Kumar ; Rakwal, Randeep ; Kang, Kyu Young. / The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants. In: Molecules and cells. 2011 ; Vol. 31, No. 1. pp. 25-31.
@article{812c0c26fef5494cb3834856d73541c2,
title = "The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants",
abstract = "Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumulate in tissues undergoing cell death. However, the function of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dosedependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the mechanism by which PBZ1 confers the cell death morphology in rice and other model plants.",
author = "Kim, {Sang Gon} and Kim, {Sun Tae} and Yiming Wang and Seok Yu and Choi, {In Soo} and Kim, {Yong Chul} and Kim, {Woo Taek} and Agrawal, {Ganesh Kumar} and Randeep Rakwal and Kang, {Kyu Young}",
year = "2011",
month = "1",
day = "1",
doi = "10.1007/s10059-011-0004-z",
language = "English",
volume = "31",
pages = "25--31",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

Kim, SG, Kim, ST, Wang, Y, Yu, S, Choi, IS, Kim, YC, Kim, WT, Agrawal, GK, Rakwal, R & Kang, KY 2011, 'The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants', Molecules and cells, vol. 31, no. 1, pp. 25-31. https://doi.org/10.1007/s10059-011-0004-z

The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants. / Kim, Sang Gon; Kim, Sun Tae; Wang, Yiming; Yu, Seok; Choi, In Soo; Kim, Yong Chul; Kim, Woo Taek; Agrawal, Ganesh Kumar; Rakwal, Randeep; Kang, Kyu Young.

In: Molecules and cells, Vol. 31, No. 1, 01.01.2011, p. 25-31.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The RNase activity of rice probenazole-induced protein1 (PBZ1) Plays a key role in cell death in plants

AU - Kim, Sang Gon

AU - Kim, Sun Tae

AU - Wang, Yiming

AU - Yu, Seok

AU - Choi, In Soo

AU - Kim, Yong Chul

AU - Kim, Woo Taek

AU - Agrawal, Ganesh Kumar

AU - Rakwal, Randeep

AU - Kang, Kyu Young

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumulate in tissues undergoing cell death. However, the function of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dosedependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the mechanism by which PBZ1 confers the cell death morphology in rice and other model plants.

AB - Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumulate in tissues undergoing cell death. However, the function of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dosedependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the mechanism by which PBZ1 confers the cell death morphology in rice and other model plants.

UR - http://www.scopus.com/inward/record.url?scp=79959377828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959377828&partnerID=8YFLogxK

U2 - 10.1007/s10059-011-0004-z

DO - 10.1007/s10059-011-0004-z

M3 - Article

C2 - 21110127

AN - SCOPUS:79959377828

VL - 31

SP - 25

EP - 31

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -