The role of IL-13 in established allergic airway disease

Christian Taube, Catherine Duez, Zhi Hua Cui, Katsuyuki Takeda, Yeong Ho Rha, Jung Won Park, Annette Balhorn, Debra D. Donaldson, Azzeddine Dakhama, Erwin W. Gelfand

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Abstract

The effectiveness of targeting IL-13 in models where airway hyperresponsiveness (AHR) and airway inflammation have already been established is not well-described. We investigated the effects of blocking IL-13 on the early and late phase airway responses and the development of AHR in previously sensitized and challenged mice. BALB/cByJ mice were sensitized (days 1 and 14) and challenged (days 28-30) with OVA. Six weeks later (day 72), previously sensitized/challenged mice were challenged with a single OVA aerosol and the early and late phase response and development of AHR were determined. Specific in vivo blockade of IL-13 was attained after i.p. injection of a soluble IL-13Rα2-IgG fusion protein (sIL-13Rα2Fc) on days 71-72 for the early and late responses and on days 71-73 for the development of AHR. sIL-13Rα2Fc administration inhibited the late, but not early, phase response and the OVA challenge-induced changes in lung resistance and dynamic compliance; as well, sIL-13Rα2Fc administration decreased bronchoalveolar lavage eosinophilia and mucus hypersecretion following the secondary challenge protocols. These results demonstrate that targeting IL-13 alone regulates airway responses when administrated to mice with established allergic airway disease. These data identify the importance of IL-13 in the development of allergen-induced altered airway responsiveness following airway challenge, even when administered before rechallenge of mice in which allergic disease had been previously established.

Original languageEnglish
Pages (from-to)6482-6489
Number of pages8
JournalJournal of Immunology
Volume169
Issue number11
DOIs
Publication statusPublished - 2002 Dec 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Taube, C., Duez, C., Cui, Z. H., Takeda, K., Rha, Y. H., Park, J. W., Balhorn, A., Donaldson, D. D., Dakhama, A., & Gelfand, E. W. (2002). The role of IL-13 in established allergic airway disease. Journal of Immunology, 169(11), 6482-6489. https://doi.org/10.4049/jimmunol.169.11.6482