The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes

S. Chu, W. Park, K. Lee, Y. Pae

Research output: Contribution to journalArticle

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Abstract

Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver diseases. The objective of this study was to determine the role of lipid peroxidation and glutathione on the bile acid-induced hepatic cell death mechanism in primary cultured rat hepatocytes. To induce hepatic cell death, we incubated primary cultured rat hepatocytes with glycochenodeoxycholic acid (GCDC; 0~400 μM) for 3 hours. In electron microscopic examination and agarose gel electrophoresis, low concentration of GCDC treatment mainly induced apoptotic feature. Whereas 400 μM GCDC treated cells demonstrated both apoptosis and necrosis. Lipid peroxidation was increased dose-dependently in GCDC treated hepatocyte. And this was also accompanied by decreased glutathione. Therefore, oxygen free radical damage may play a partial role in GCDC-induced hepatic cell death.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalKorean Journal of Physiology and Pharmacology
Volume4
Issue number2
Publication statusPublished - 2000 Jan 1

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Glycochenodeoxycholic Acid
Lipid Peroxidation
Glutathione
Hepatocytes
Cell Death
Bile Acids and Salts
Agar Gel Electrophoresis
Cholestasis
Free Radicals
Liver Diseases
Reactive Oxygen Species
Necrosis
Electrons
Apoptosis

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

Cite this

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abstract = "Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver diseases. The objective of this study was to determine the role of lipid peroxidation and glutathione on the bile acid-induced hepatic cell death mechanism in primary cultured rat hepatocytes. To induce hepatic cell death, we incubated primary cultured rat hepatocytes with glycochenodeoxycholic acid (GCDC; 0~400 μM) for 3 hours. In electron microscopic examination and agarose gel electrophoresis, low concentration of GCDC treatment mainly induced apoptotic feature. Whereas 400 μM GCDC treated cells demonstrated both apoptosis and necrosis. Lipid peroxidation was increased dose-dependently in GCDC treated hepatocyte. And this was also accompanied by decreased glutathione. Therefore, oxygen free radical damage may play a partial role in GCDC-induced hepatic cell death.",
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The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes. / Chu, S.; Park, W.; Lee, K.; Pae, Y.

In: Korean Journal of Physiology and Pharmacology, Vol. 4, No. 2, 01.01.2000, p. 121-127.

Research output: Contribution to journalArticle

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