The role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling

Onju Ham, Byeong Wook Song, Se Yeon Lee, Eunmi Choi, Min Ji Cha, Chang Youn Lee, Jun Hee Park, Il Kwon Kim, Woochul Chang, Soyeon Lim, Chang Hyun Lee, Soonhag Kim, Yangsoo Jang, Ki Chul Hwang

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Abstract

Chondrogenic differentiation of mesenchymal stem cells (MSCs) is critical for successful cartilage regeneration Several methods have been developed to attempt to chondrogenic differentiation, because chondrogenic differentiated cells can form stable cartilage and induce expression of a cartilage-specific phenotype In this study, we found that both H-89 and microRNA-23b induced differentiation into chondrocyte of hMSCs through down-regulation of protein kinase A (PKA) signaling The small molecule, H-89, was identified by PCA analysis as a potential mediator of chondrogenic differentiation H-89 induced the expression of the chondrocyte marker, aggrecan, as well as miR-23b We searched that miR-23b regulates protein level of PKA When miR-23b was transfected into hMSCs, chondrogenic differentiation was induced We confirmed the target of miR-23b using a reporter gene assay Furthermore, not only H-89 or miR-23b-treated cells, but also cell co-treated with H-89 and miR-23b differentiated into chondrocytes Our results indicate that H-89 induces the expression of endogenous miR-23b, thereby inducing chondrogenic differentiation by negatively inhibition of PKA signaling

Original languageEnglish
Pages (from-to)4500-4507
Number of pages8
JournalBiomaterials
Volume33
Issue number18
DOIs
Publication statusPublished - 2012 Jun 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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    Ham, O., Song, B. W., Lee, S. Y., Choi, E., Cha, M. J., Lee, C. Y., Park, J. H., Kim, I. K., Chang, W., Lim, S., Lee, C. H., Kim, S., Jang, Y., & Hwang, K. C. (2012). The role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling. Biomaterials, 33(18), 4500-4507. https://doi.org/10.1016/j.biomaterials.2012.03.025