The roles of reactive oxygen species produced by contact allergens and irritants in monocyte-derived dendritic cells

Dashlkhumbe Byamba, Tae Gyun Kim, Dong Hyun Kim, Jeong Hwan Je, Mingeol Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Although reactive oxygen species (ROS) have been produced in both mouse bone marrow-derived dendritic cells (DCs) and XS-106 DCs by contact sensitizers and irritants in previous studies, the generation of ROS in human monocyte-derived DCs (MoDCs) and their role in contact hypersensitivity (CHS) has yet to be elucidated. Objective: The purpose of this study was to determine whether contact allergens and irritants induce ROS in MoDCs and, if so, to evaluate the role of contact al lergen and irritant induced-ROS in MoDCs in CHS. Methods: Production of ROS was measured by 5-(and-6)-chloromethyl-2',7'- dichlorodihydrofluoresceln diacetate (CMH2DCFDA) assay. Surface CD86 and HLA-DR molecules were detected by flow cytometry. Protein carbonylation was detected by Western blotting. Results: ROS were produced by contact allergens such as dinitrochlorobenzene (DNCB) and thimerosal and the irritant benzalkonium chloride (BKC). DNCB-induced, but not BKC-induced, ROS increased surface CD86 and HLA-DR molecules on MoDCs and induced protein carbonylation. These changes were reduced in the presence of antioxidant N-acetyl cysteine. Conclusion: Our results suggest that DNCB-induced ROS may be different from those induced by irritant BKC. The DNCB-induced ROS may be associated with the CHS response, because they activate surface molecules on DCs that are important for generating immune reactions.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalAnnals of Dermatology
Volume22
Issue number3
DOIs
Publication statusPublished - 2010 Aug 1

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Irritants
Allergens
Dendritic Cells
Monocytes
Reactive Oxygen Species
Dinitrochlorobenzene
Benzalkonium Compounds
Contact Dermatitis
Protein Carbonylation
HLA-DR Antigens
Thimerosal
Cysteine
Flow Cytometry
Antioxidants
Bone Marrow
Western Blotting

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Byamba, Dashlkhumbe ; Kim, Tae Gyun ; Kim, Dong Hyun ; Je, Jeong Hwan ; Lee, Mingeol. / The roles of reactive oxygen species produced by contact allergens and irritants in monocyte-derived dendritic cells. In: Annals of Dermatology. 2010 ; Vol. 22, No. 3. pp. 269-278.
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The roles of reactive oxygen species produced by contact allergens and irritants in monocyte-derived dendritic cells. / Byamba, Dashlkhumbe; Kim, Tae Gyun; Kim, Dong Hyun; Je, Jeong Hwan; Lee, Mingeol.

In: Annals of Dermatology, Vol. 22, No. 3, 01.08.2010, p. 269-278.

Research output: Contribution to journalArticle

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N2 - Background: Although reactive oxygen species (ROS) have been produced in both mouse bone marrow-derived dendritic cells (DCs) and XS-106 DCs by contact sensitizers and irritants in previous studies, the generation of ROS in human monocyte-derived DCs (MoDCs) and their role in contact hypersensitivity (CHS) has yet to be elucidated. Objective: The purpose of this study was to determine whether contact allergens and irritants induce ROS in MoDCs and, if so, to evaluate the role of contact al lergen and irritant induced-ROS in MoDCs in CHS. Methods: Production of ROS was measured by 5-(and-6)-chloromethyl-2',7'- dichlorodihydrofluoresceln diacetate (CMH2DCFDA) assay. Surface CD86 and HLA-DR molecules were detected by flow cytometry. Protein carbonylation was detected by Western blotting. Results: ROS were produced by contact allergens such as dinitrochlorobenzene (DNCB) and thimerosal and the irritant benzalkonium chloride (BKC). DNCB-induced, but not BKC-induced, ROS increased surface CD86 and HLA-DR molecules on MoDCs and induced protein carbonylation. These changes were reduced in the presence of antioxidant N-acetyl cysteine. Conclusion: Our results suggest that DNCB-induced ROS may be different from those induced by irritant BKC. The DNCB-induced ROS may be associated with the CHS response, because they activate surface molecules on DCs that are important for generating immune reactions.

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