The stress-activated MAP kinase Sty1/Spc1 and a 3'-regulatory element mediate UV-induced expression of the uvi15+ gene at the post-transcriptional level

Minkyu Kim, Woojin Lee, Jiyoung Park, Jae Bum Kim, Yeun Kyu Jang, Rho Hyun Seong, Soo Young Choe, Sang Dai Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Exposure of Schizosaccharomyces pombe cells to UV light results in increased uvi15+ gene expression at both the mRNA and protein levels, leading to elevated cell survival. This UV-induced expression of the uvi15+ gene was reduced in Δsty1 and Δwis1 cells lacking the stress-activated protein kinase pathway, but not in DNA damage checkpoint mutants. To further understand the cellular mechanisms responsible for this UV-induced expression, the transcription rate and mRNA half-life were investigated. Transcription run-on assays revealed that the rate of uvi15+ transcription was increased 1.8-fold regardless of Sty1 when cells were UV irradiated. The half-life of uvi15+ mRNA was also increased 1.5-fold after UV irradiation, but it was decreased in the Δsty1 background for both basal and UV-induced mRNAs, indicating that the stress-activated MAPK cascade can mediate UV-induced gene expression by increasing mRNA half-life. Deletion analyses identified a 54 nt element downstream of the distal poly(A) site, which was involved in the increased half-life of uvi15+ mRNA. These results suggest that both Sty1 and the 3'-regulatory element regulate UV-induced expression of the uvi15+ gene at the post-transcriptional level.

Original languageEnglish
Pages (from-to)3392-3402
Number of pages11
JournalNucleic acids research
Volume28
Issue number17
Publication statusPublished - 2000 Sep 1

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Phosphotransferases
Gene Expression
Messenger RNA
Half-Life
Poly A
Schizosaccharomyces
Ultraviolet Rays
Heat-Shock Proteins
Protein Kinases
DNA Damage
Cell Survival
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Kim, Minkyu ; Lee, Woojin ; Park, Jiyoung ; Kim, Jae Bum ; Jang, Yeun Kyu ; Seong, Rho Hyun ; Choe, Soo Young ; Park, Sang Dai. / The stress-activated MAP kinase Sty1/Spc1 and a 3'-regulatory element mediate UV-induced expression of the uvi15+ gene at the post-transcriptional level. In: Nucleic acids research. 2000 ; Vol. 28, No. 17. pp. 3392-3402.
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abstract = "Exposure of Schizosaccharomyces pombe cells to UV light results in increased uvi15+ gene expression at both the mRNA and protein levels, leading to elevated cell survival. This UV-induced expression of the uvi15+ gene was reduced in Δsty1 and Δwis1 cells lacking the stress-activated protein kinase pathway, but not in DNA damage checkpoint mutants. To further understand the cellular mechanisms responsible for this UV-induced expression, the transcription rate and mRNA half-life were investigated. Transcription run-on assays revealed that the rate of uvi15+ transcription was increased 1.8-fold regardless of Sty1 when cells were UV irradiated. The half-life of uvi15+ mRNA was also increased 1.5-fold after UV irradiation, but it was decreased in the Δsty1 background for both basal and UV-induced mRNAs, indicating that the stress-activated MAPK cascade can mediate UV-induced gene expression by increasing mRNA half-life. Deletion analyses identified a 54 nt element downstream of the distal poly(A) site, which was involved in the increased half-life of uvi15+ mRNA. These results suggest that both Sty1 and the 3'-regulatory element regulate UV-induced expression of the uvi15+ gene at the post-transcriptional level.",
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The stress-activated MAP kinase Sty1/Spc1 and a 3'-regulatory element mediate UV-induced expression of the uvi15+ gene at the post-transcriptional level. / Kim, Minkyu; Lee, Woojin; Park, Jiyoung; Kim, Jae Bum; Jang, Yeun Kyu; Seong, Rho Hyun; Choe, Soo Young; Park, Sang Dai.

In: Nucleic acids research, Vol. 28, No. 17, 01.09.2000, p. 3392-3402.

Research output: Contribution to journalArticle

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AU - Kim, Minkyu

AU - Lee, Woojin

AU - Park, Jiyoung

AU - Kim, Jae Bum

AU - Jang, Yeun Kyu

AU - Seong, Rho Hyun

AU - Choe, Soo Young

AU - Park, Sang Dai

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N2 - Exposure of Schizosaccharomyces pombe cells to UV light results in increased uvi15+ gene expression at both the mRNA and protein levels, leading to elevated cell survival. This UV-induced expression of the uvi15+ gene was reduced in Δsty1 and Δwis1 cells lacking the stress-activated protein kinase pathway, but not in DNA damage checkpoint mutants. To further understand the cellular mechanisms responsible for this UV-induced expression, the transcription rate and mRNA half-life were investigated. Transcription run-on assays revealed that the rate of uvi15+ transcription was increased 1.8-fold regardless of Sty1 when cells were UV irradiated. The half-life of uvi15+ mRNA was also increased 1.5-fold after UV irradiation, but it was decreased in the Δsty1 background for both basal and UV-induced mRNAs, indicating that the stress-activated MAPK cascade can mediate UV-induced gene expression by increasing mRNA half-life. Deletion analyses identified a 54 nt element downstream of the distal poly(A) site, which was involved in the increased half-life of uvi15+ mRNA. These results suggest that both Sty1 and the 3'-regulatory element regulate UV-induced expression of the uvi15+ gene at the post-transcriptional level.

AB - Exposure of Schizosaccharomyces pombe cells to UV light results in increased uvi15+ gene expression at both the mRNA and protein levels, leading to elevated cell survival. This UV-induced expression of the uvi15+ gene was reduced in Δsty1 and Δwis1 cells lacking the stress-activated protein kinase pathway, but not in DNA damage checkpoint mutants. To further understand the cellular mechanisms responsible for this UV-induced expression, the transcription rate and mRNA half-life were investigated. Transcription run-on assays revealed that the rate of uvi15+ transcription was increased 1.8-fold regardless of Sty1 when cells were UV irradiated. The half-life of uvi15+ mRNA was also increased 1.5-fold after UV irradiation, but it was decreased in the Δsty1 background for both basal and UV-induced mRNAs, indicating that the stress-activated MAPK cascade can mediate UV-induced gene expression by increasing mRNA half-life. Deletion analyses identified a 54 nt element downstream of the distal poly(A) site, which was involved in the increased half-life of uvi15+ mRNA. These results suggest that both Sty1 and the 3'-regulatory element regulate UV-induced expression of the uvi15+ gene at the post-transcriptional level.

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