The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole

Hae Sun Chung, Kyeongmi Kim, Sang Sook Hong, Seong Geun Hong, Kyungwon Lee, Yunsop Chong

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Emerging resistance to trimethoprim/sulfamethoxazole (SXT) poses a serious threat to the treatment of Stenotrophomonas maltophilia infections. We determined the prevalence and molecular characteristics of acquired SXT resistance in recent clinical S. maltophilia isolates obtained from Korea. A total of 252 clinical isolates of S. maltophilia were collected from 10 university hospitals in Korea between 2009 and 2010. Antimicrobial susceptibility was determined by using the CLSI agar dilution method. The sul1, sul2, and sul3 genes, integrons, insertion sequence common region (ISCR) elements, and dfrA genes were detected using PCR. The presence of the sul1 gene and integrons was confirmed through sequence analysis. Among the 32 SXT-resistant isolates, sul1 was detected in 23 isolates (72%), all of which demonstrated high-level resistance (≥64 mg/L) to SXT. The sul1 gene (varying in size and structure) was linked to class 1 integrons in 15 of the 23 isolates (65%) harboring this gene. None of the SXT-susceptible isolates or the SXT-resistant isolates with a minimum inhibitory concentration of 4 and 8 mg/L were positive for sul1. Moreover, the sul2, sul3, and dfrA genes or the ISCR elements were not detected. The sul1 gene may play an important role in the high-level SXT resistance observed in S. maltophilia.

Original languageEnglish
Pages (from-to)246-249
Number of pages4
JournalAnnals of laboratory medicine
Volume35
Issue number2
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Stenotrophomonas maltophilia
Sulfamethoxazole Drug Combination Trimethoprim
Integrons
Genes
Insertional Mutagenesis
Korea
Microbial Sensitivity Tests
Agar
Sequence Analysis
Dilution
Polymerase Chain Reaction
Infection

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Chung, Hae Sun ; Kim, Kyeongmi ; Hong, Sang Sook ; Hong, Seong Geun ; Lee, Kyungwon ; Chong, Yunsop. / The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole. In: Annals of laboratory medicine. 2015 ; Vol. 35, No. 2. pp. 246-249.
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The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole. / Chung, Hae Sun; Kim, Kyeongmi; Hong, Sang Sook; Hong, Seong Geun; Lee, Kyungwon; Chong, Yunsop.

In: Annals of laboratory medicine, Vol. 35, No. 2, 01.01.2015, p. 246-249.

Research output: Contribution to journalArticle

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