The survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy

Sun Woong Kim, Hwan Young Lee, Tae Im Kim, Kyoung Jin Shin, Woo Ick Yang, Eung Kweon Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Aims: To investigate the survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy. Methods: In 1996, a 48-year-old male with lattice corneal dystrophy underwent penetrating keratoplasty 3 times in the right eye within a 3-month interval. Nine years and 7 months later, the patient underwent a fourth penetrating keratoplasty. After surgery, the previous graft was analyzed to determine the origin of the cells. The epithelium and endothelium were removed, and then the button was dissected into 5 stromal blocks measuring 2 × 1.8 mm. Tissues underwent forensic genotyping using 16 markers (amelogenin for sex chromosomes and 15 autosomal short tandem repeats). Patient buccal tissue DNA was used as a control. Results: The epithelium and buccal tissue contained identical DNA (i.e. recipient DNA). Similarly, the most peripheral stromal tissue contained only recipient DNA. In contrast, the most central stromal tissue only contained DNA of nonrecipient origin (presumably donor), while the stromal tissue between the periphery and center contained both recipient and nonrecipient DNA. Conclusions: The corneal stroma was infiltrated by surrounding recipient-derived keratocytes from the periphery. Therefore, more donor-derived cells had survived in the central stroma.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalOphthalmologica
Volume223
Issue number6
DOIs
Publication statusPublished - 2009 Oct

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems

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