The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture

Joohyeon Hong, Jungryun Lee, Kiyeong Song, Go Eun Ha, Yong Ryoul Yang, Ji Su Ma, Masahiro Yamamoto, Hee Sup Shin, Pann Ghill Suh, Eunji Cheong

Research output: Contribution to journalArticle

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Abstract

The transition from wakefulness to a nonrapid eye movement (NREM) sleep state at the onset of sleep involves a transition from low-voltage, high-frequency irregular electroencephalography (EEG) waveforms to large-amplitude, low-frequency EEG waveforms accompanying synchronized oscillatory activity in the thalamocortical circuit. The thalamocortical circuit consists of reciprocal connections between the thalamus and cortex. The cortex sends strong excitatory feedback to the thalamus, however the function of which is unclear. In this study, we investigated the role of the thalamic metabotropic glutamate receptor 1 (mGluR1)-phospholipase C β4 (PLCβ4) pathway in sleep control in PLCβ4-deficient (PLCβ4-/-) mice. The thalamic mGluR1-PLCβ4 pathway contains synapses that receive corticothalamic inputs. In PLCβ4-/- mice, the transition from wakefulness to the NREM sleep state was stimulated, and the NREM sleep state was stabilized, which resulted in increased NREM sleep. The power density of delta (δ) waves increased in parallel with the increased NREM sleep. These sleep phenotypes in PLCβ4-/- mice were consistent in TC-restricted PLCβ4 knockdown mice. Moreover, in vitro intrathalamic oscillations were greatly enhanced in the PLCβ4-/- slices. The results of our study showed that thalamic mGluR1-PLCβ4 pathway was critical in controlling sleep architecture.

Original languageEnglish
Article number100
JournalMolecular Brain
Volume9
Issue number1
DOIs
Publication statusPublished - 2016 Dec 21

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Critical Pathways
Type C Phospholipases
Sleep
Eye Movements
Wakefulness
Thalamus
Electroencephalography
metabotropic glutamate receptor type 1
Synapses
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Hong, Joohyeon ; Lee, Jungryun ; Song, Kiyeong ; Ha, Go Eun ; Yang, Yong Ryoul ; Ma, Ji Su ; Yamamoto, Masahiro ; Shin, Hee Sup ; Suh, Pann Ghill ; Cheong, Eunji. / The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture. In: Molecular Brain. 2016 ; Vol. 9, No. 1.
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Hong, J, Lee, J, Song, K, Ha, GE, Yang, YR, Ma, JS, Yamamoto, M, Shin, HS, Suh, PG & Cheong, E 2016, 'The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture', Molecular Brain, vol. 9, no. 1, 100. https://doi.org/10.1186/s13041-016-0276-5

The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture. / Hong, Joohyeon; Lee, Jungryun; Song, Kiyeong; Ha, Go Eun; Yang, Yong Ryoul; Ma, Ji Su; Yamamoto, Masahiro; Shin, Hee Sup; Suh, Pann Ghill; Cheong, Eunji.

In: Molecular Brain, Vol. 9, No. 1, 100, 21.12.2016.

Research output: Contribution to journalArticle

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AU - Hong, Joohyeon

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AU - Ma, Ji Su

AU - Yamamoto, Masahiro

AU - Shin, Hee Sup

AU - Suh, Pann Ghill

AU - Cheong, Eunji

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