Abstract
Osteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.
| Original language | English |
|---|---|
| Pages (from-to) | 1498-1507 |
| Number of pages | 10 |
| Journal | Bone |
| Volume | 46 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2010 Jun 1 |
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All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Physiology
- Histology
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The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression. / Park, Su Jin; Jung, Seung Hyun; Jogeswar, Gadi; Ryoo, Hyun Mo; Yook, Jong In; Choi, Han Seok; Rhee, Yumie; Kim, Cheol Hee; Lim, Sung Kil.
In: Bone, Vol. 46, No. 6, 01.06.2010, p. 1498-1507.Research output: Contribution to journal › Article
TY - JOUR
T1 - The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression
AU - Park, Su Jin
AU - Jung, Seung Hyun
AU - Jogeswar, Gadi
AU - Ryoo, Hyun Mo
AU - Yook, Jong In
AU - Choi, Han Seok
AU - Rhee, Yumie
AU - Kim, Cheol Hee
AU - Lim, Sung Kil
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Osteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.
AB - Osteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.
UR - http://www.scopus.com/inward/record.url?scp=77953026325&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953026325&partnerID=8YFLogxK
U2 - 10.1016/j.bone.2010.02.027
DO - 10.1016/j.bone.2010.02.027
M3 - Article
C2 - 20215006
AN - SCOPUS:77953026325
VL - 46
SP - 1498
EP - 1507
JO - Bone
JF - Bone
SN - 8756-3282
IS - 6
ER -