The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression

Su Jin Park, Seung Hyun Jung, Gadi Jogeswar, Hyun Mo Ryoo, Jong In Yook, Han Seok Choi, Yumie Rhee, Cheol Hee Kim, Sung Kil Lim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Osteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.

Original languageEnglish
Pages (from-to)1498-1507
Number of pages10
JournalBone
Volume46
Issue number6
DOIs
Publication statusPublished - 2010 Jun

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

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    Park, S. J., Jung, S. H., Jogeswar, G., Ryoo, H. M., Yook, J. I., Choi, H. S., Rhee, Y., Kim, C. H., & Lim, S. K. (2010). The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression. Bone, 46(6), 1498-1507. https://doi.org/10.1016/j.bone.2010.02.027