The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression

Su Jin Park, Seung Hyun Jung, Gadi Jogeswar, Hyun Mo Ryoo, Jong In Yook, Han Seok Choi, Yumie Rhee, Cheol Hee Kim, Sung Kil Lim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Osteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.

Original languageEnglish
Pages (from-to)1498-1507
Number of pages10
JournalBone
Volume46
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Osteoblasts
Transcription Factors
Snails
E-Box Elements
Morpholinos
Epithelial-Mesenchymal Transition
Antisense Oligonucleotides
Zinc Fingers
Zebrafish
Mesenchymal Stromal Cells
Skeleton
Embryonic Development
Genes
Snail Family Transcription Factors
Neoplasms

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Park, S. J., Jung, S. H., Jogeswar, G., Ryoo, H. M., Yook, J. I., Choi, H. S., ... Lim, S. K. (2010). The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression. Bone, 46(6), 1498-1507. https://doi.org/10.1016/j.bone.2010.02.027
Park, Su Jin ; Jung, Seung Hyun ; Jogeswar, Gadi ; Ryoo, Hyun Mo ; Yook, Jong In ; Choi, Han Seok ; Rhee, Yumie ; Kim, Cheol Hee ; Lim, Sung Kil. / The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression. In: Bone. 2010 ; Vol. 46, No. 6. pp. 1498-1507.
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Park, SJ, Jung, SH, Jogeswar, G, Ryoo, HM, Yook, JI, Choi, HS, Rhee, Y, Kim, CH & Lim, SK 2010, 'The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression', Bone, vol. 46, no. 6, pp. 1498-1507. https://doi.org/10.1016/j.bone.2010.02.027

The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression. / Park, Su Jin; Jung, Seung Hyun; Jogeswar, Gadi; Ryoo, Hyun Mo; Yook, Jong In; Choi, Han Seok; Rhee, Yumie; Kim, Cheol Hee; Lim, Sung Kil.

In: Bone, Vol. 46, No. 6, 01.06.2010, p. 1498-1507.

Research output: Contribution to journalArticle

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AU - Jung, Seung Hyun

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AU - Yook, Jong In

AU - Choi, Han Seok

AU - Rhee, Yumie

AU - Kim, Cheol Hee

AU - Lim, Sung Kil

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