The transcriptional cofactor TRIM33 prevents apoptosis in B lymphoblastic leukemia by deactivating a single enhancer

Eric Wang, Shinpei Kawaoka, Jae-Seok Roe, Junwei Shi, Anja F. Hohmann, Yali Xu, Anand S. Bhagwat, Yutaka Suzuki, Justin B. Kinney, Christopher R. Vakoc

Research output: Contribution to journalArticle

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Abstract

Most mammalian transcription factors (TFs) and cofactors occupy thousands of genomic sites and modulate the expression of large gene networks to implement their biological functions. In this study, we describe an exception to this paradigm. TRIM33 is identified here as a lineage dependency in B cell neoplasms and is shown to perform this essential function by associating with a single cis element. ChIP-seq analysis of TRIM33 in murine B cell leukemia revealed a preferential association with two lineage-specific enhancers that harbor an exceptional density of motifs recognized by the PU.1 TF. TRIM33 is recruited to these elements by PU.1, yet acts to antagonize PU.1 function. One of the PU.1/TRIM33 co-occupied enhancers is upstream of the pro-apoptotic gene Bim, and deleting this enhancer renders TRIM33 dispensable for leukemia cell survival. These findings reveal an essential role for TRIM33 in preventing apoptosis in B lymphoblastic leukemia by interfering with enhancer-mediated Bim activation.

Original languageEnglish
Pages (from-to)e06377
JournaleLife
Volume4
DOIs
Publication statusPublished - 2015 Apr 28

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
Transcription Factors
Cells
B-Cell Leukemia
Apoptosis
Gene Regulatory Networks
Genes
Cell Survival
Leukemia
B-Lymphocytes
Ports and harbors
Chemical activation
Association reactions
Neoplasms

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Wang, Eric ; Kawaoka, Shinpei ; Roe, Jae-Seok ; Shi, Junwei ; Hohmann, Anja F. ; Xu, Yali ; Bhagwat, Anand S. ; Suzuki, Yutaka ; Kinney, Justin B. ; Vakoc, Christopher R. / The transcriptional cofactor TRIM33 prevents apoptosis in B lymphoblastic leukemia by deactivating a single enhancer. In: eLife. 2015 ; Vol. 4. pp. e06377.
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Wang, E, Kawaoka, S, Roe, J-S, Shi, J, Hohmann, AF, Xu, Y, Bhagwat, AS, Suzuki, Y, Kinney, JB & Vakoc, CR 2015, 'The transcriptional cofactor TRIM33 prevents apoptosis in B lymphoblastic leukemia by deactivating a single enhancer', eLife, vol. 4, pp. e06377. https://doi.org/10.7554/eLife.06377

The transcriptional cofactor TRIM33 prevents apoptosis in B lymphoblastic leukemia by deactivating a single enhancer. / Wang, Eric; Kawaoka, Shinpei; Roe, Jae-Seok; Shi, Junwei; Hohmann, Anja F.; Xu, Yali; Bhagwat, Anand S.; Suzuki, Yutaka; Kinney, Justin B.; Vakoc, Christopher R.

In: eLife, Vol. 4, 28.04.2015, p. e06377.

Research output: Contribution to journalArticle

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AU - Roe, Jae-Seok

AU - Shi, Junwei

AU - Hohmann, Anja F.

AU - Xu, Yali

AU - Bhagwat, Anand S.

AU - Suzuki, Yutaka

AU - Kinney, Justin B.

AU - Vakoc, Christopher R.

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