The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria

Joseph Jeong, Sung Ryul Kim, Seon Ho Lee, Ji Hun Lim, Jung In Choi, Jae Sun Park, Chulhun L. Chang, Jun Yong Choi, Douglas D. Richman, Davey M. Smith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: We evaluated high-performance liquid chromatography (HPLC) for species identification of mycobacteria from various clinical specimens in an urban hospital in South Korea between January 2005 and December 2009. Methods: In the study period 24,774 cultures were completed, yielding the 3215 clinical isolates cultivated for mycobacteria and positive cultures that had mycolic acid investigated by HPLC. For species identification, we compared HPLC patterns of clinical isolates with 33 standard Mycobacterium species. Results: There were 3 different HPLC groups with single, double, and triple-cluster patterns representing 9, 20, and 4 mycobacterial species, respectively. Species identification rates of HPLC for Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) were found to be 100% and 95.6%, respectively. Among mycobacterial isolates, 12.1% were NTM-positive. There were 20 different NTM species with frequencies of 0.3%~15.5%. Conclusion: The HPLC method was highly sensitive identifying NTM isolated from clinical specimens.

Original languageEnglish
Pages (from-to)612-617
Number of pages6
JournalLaboratory medicine
Volume42
Issue number10
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Epidemiology
High performance liquid chromatography
Mycobacterium
Nontuberculous Mycobacteria
High Pressure Liquid Chromatography
Mycolic Acids
Republic of Korea
Urban Hospitals
Mycobacterium tuberculosis

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Jeong, J., Kim, S. R., Lee, S. H., Lim, J. H., Choi, J. I., Park, J. S., ... Smith, D. M. (2011). The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria. Laboratory medicine, 42(10), 612-617. https://doi.org/10.1309/LMDDEHPSYE6ZDM3C
Jeong, Joseph ; Kim, Sung Ryul ; Lee, Seon Ho ; Lim, Ji Hun ; Choi, Jung In ; Park, Jae Sun ; Chang, Chulhun L. ; Choi, Jun Yong ; Richman, Douglas D. ; Smith, Davey M. / The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria. In: Laboratory medicine. 2011 ; Vol. 42, No. 10. pp. 612-617.
@article{0848b85f90dd405ca7652d563c518343,
title = "The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria",
abstract = "Objective: We evaluated high-performance liquid chromatography (HPLC) for species identification of mycobacteria from various clinical specimens in an urban hospital in South Korea between January 2005 and December 2009. Methods: In the study period 24,774 cultures were completed, yielding the 3215 clinical isolates cultivated for mycobacteria and positive cultures that had mycolic acid investigated by HPLC. For species identification, we compared HPLC patterns of clinical isolates with 33 standard Mycobacterium species. Results: There were 3 different HPLC groups with single, double, and triple-cluster patterns representing 9, 20, and 4 mycobacterial species, respectively. Species identification rates of HPLC for Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) were found to be 100{\%} and 95.6{\%}, respectively. Among mycobacterial isolates, 12.1{\%} were NTM-positive. There were 20 different NTM species with frequencies of 0.3{\%}~15.5{\%}. Conclusion: The HPLC method was highly sensitive identifying NTM isolated from clinical specimens.",
author = "Joseph Jeong and Kim, {Sung Ryul} and Lee, {Seon Ho} and Lim, {Ji Hun} and Choi, {Jung In} and Park, {Jae Sun} and Chang, {Chulhun L.} and Choi, {Jun Yong} and Richman, {Douglas D.} and Smith, {Davey M.}",
year = "2011",
month = "10",
day = "1",
doi = "10.1309/LMDDEHPSYE6ZDM3C",
language = "English",
volume = "42",
pages = "612--617",
journal = "Laboratory Medicine",
issn = "0007-5027",
publisher = "American Society of Clinical Pathologists",
number = "10",

}

Jeong, J, Kim, SR, Lee, SH, Lim, JH, Choi, JI, Park, JS, Chang, CL, Choi, JY, Richman, DD & Smith, DM 2011, 'The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria', Laboratory medicine, vol. 42, no. 10, pp. 612-617. https://doi.org/10.1309/LMDDEHPSYE6ZDM3C

The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria. / Jeong, Joseph; Kim, Sung Ryul; Lee, Seon Ho; Lim, Ji Hun; Choi, Jung In; Park, Jae Sun; Chang, Chulhun L.; Choi, Jun Yong; Richman, Douglas D.; Smith, Davey M.

In: Laboratory medicine, Vol. 42, No. 10, 01.10.2011, p. 612-617.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria

AU - Jeong, Joseph

AU - Kim, Sung Ryul

AU - Lee, Seon Ho

AU - Lim, Ji Hun

AU - Choi, Jung In

AU - Park, Jae Sun

AU - Chang, Chulhun L.

AU - Choi, Jun Yong

AU - Richman, Douglas D.

AU - Smith, Davey M.

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Objective: We evaluated high-performance liquid chromatography (HPLC) for species identification of mycobacteria from various clinical specimens in an urban hospital in South Korea between January 2005 and December 2009. Methods: In the study period 24,774 cultures were completed, yielding the 3215 clinical isolates cultivated for mycobacteria and positive cultures that had mycolic acid investigated by HPLC. For species identification, we compared HPLC patterns of clinical isolates with 33 standard Mycobacterium species. Results: There were 3 different HPLC groups with single, double, and triple-cluster patterns representing 9, 20, and 4 mycobacterial species, respectively. Species identification rates of HPLC for Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) were found to be 100% and 95.6%, respectively. Among mycobacterial isolates, 12.1% were NTM-positive. There were 20 different NTM species with frequencies of 0.3%~15.5%. Conclusion: The HPLC method was highly sensitive identifying NTM isolated from clinical specimens.

AB - Objective: We evaluated high-performance liquid chromatography (HPLC) for species identification of mycobacteria from various clinical specimens in an urban hospital in South Korea between January 2005 and December 2009. Methods: In the study period 24,774 cultures were completed, yielding the 3215 clinical isolates cultivated for mycobacteria and positive cultures that had mycolic acid investigated by HPLC. For species identification, we compared HPLC patterns of clinical isolates with 33 standard Mycobacterium species. Results: There were 3 different HPLC groups with single, double, and triple-cluster patterns representing 9, 20, and 4 mycobacterial species, respectively. Species identification rates of HPLC for Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) were found to be 100% and 95.6%, respectively. Among mycobacterial isolates, 12.1% were NTM-positive. There were 20 different NTM species with frequencies of 0.3%~15.5%. Conclusion: The HPLC method was highly sensitive identifying NTM isolated from clinical specimens.

UR - http://www.scopus.com/inward/record.url?scp=81455140016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81455140016&partnerID=8YFLogxK

U2 - 10.1309/LMDDEHPSYE6ZDM3C

DO - 10.1309/LMDDEHPSYE6ZDM3C

M3 - Article

AN - SCOPUS:81455140016

VL - 42

SP - 612

EP - 617

JO - Laboratory Medicine

JF - Laboratory Medicine

SN - 0007-5027

IS - 10

ER -