The XFEL protein crystallography: Developments and perspectives

Haiguang Liu, Weontae Lee

Research output: Contribution to journalEditorialpeer-review

4 Citations (Scopus)
Original languageEnglish
Article number3421
JournalInternational journal of molecular sciences
Volume20
Issue number14
DOIs
Publication statusPublished - 2019 Jul 2

Bibliographical note

Funding Information:
process: high energy electrons produce coherent X-ray pulses that intercept microcrystals delivered interpretation, i.e., the inverse problem, to be solved using computational modeling methods. The via the injecting method, resulting in diffraction signals. The lower panel shows the data analysis and pumping laser (purple dashed line) can be included for time-resolved experiments (Figure reproduced model interpretation, i.e., the inverse problem, to be solved using computational modeling methods. from [24] with permission). The pumping laser (purple dashed line) can be included for time-resolved experiments (Figure reproduced from [24] with permission). Author Contributions: Both H.L. and W.L. contributed to the writing of the manuscript. There are four essential components in XFEL protein crystallography: (1) batched microcrystal Funding: This work was funded by the National Natural Science Foundation of China grant No. 11811540392, 11575021, U1530401 to H.L. and National Research Foundation of Korea grant No. NRF-2017R1A2B2008483 toWdiffr.L. action data collection; and (4) data analysis and model interpretation. Four articles nicely cover ConcfloicmtspoofnIenntetrse(s1t:),T(h2e) aauntdh o(r4s).dTechlear seanmopcolen fdliecltiovfeirnyt emreestt.hods are summarized by Nam, with a focus on the sample delivery medium that carries the crystals to the XFEL interaction volume [14]. The sample Refperreenpcaersations are well presented in the articles on XFEL applications in determining the structure and dynamics of the HIV integrase catalytic core domain (CCD) [15] and the HIV-1 Gag matrix domain 1. Bostedt, C.; Boutet, S.; Fritz, D.M.; Huang, Z.; Lee, H.J.; Lemke, H.T.; Robert, A.; Schlotter, W.F.; Turner, J.J.; with inositol hexaphosphate (MA-IP6) [16]. XFEL protein crystallography not only determines high Williams, G.J. Linac Coherent Light Source: The first five years. Rev. Mod. Phys. 2016, 88, 015007. [CrossRef] resolution structures of proteins, but also reveals the time-stamped conformational changes of proteins. Schmidt reviews the time-resolved SFX using XFELs to study the ultrafast dynamics of protein molecules [17]. Data analysis has been a challenging task for time-resolved SFX because of

Funding Information:
This work was funded by the National Natural Science Foundation of China grant No. 11811540392, 11575021, U1530401 to H.L. and National Research Foundation of Korea grant No. NRF-2017R1A2B2008483 to W.L.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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