Therapeutic angiogenesis by a myoblast layer harvested by tissue transfer printing from cell-adhesive, thermosensitive hydrogels

Dong Wan Kim, Indong Jun, Tae Jin Lee, Ji hye Lee, Young Jun Lee, Hyeon Ki Jang, Seokyung Kang, Ki Dong Park, Seung Woo Cho, Byung Soo Kim, Heungsoo Shin

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Abstract

Peripheral arterial disease (PAD) is characterized by the altered structure and function of arteries caused by accumulated plaque. There have been many studies on treating this disease by the direct injection of various types of therapeutic cells, however, the low cell engraftment efficiency and diffusion of the transplanted cells have been major problems. In this study, we developed an approach (transfer printing) to deliver monolayer of cells to the hindlimb ischemic tissue using thermosensitive hydrogels, and investigated its efficacy in long term retention upon transplantation and therapeutic angiogenesis. We first investigated the invitro maintenance of robust cell-cell contacts and stable expression of the ECM proteins in myoblast layer following transfer printing process. In order to confirm the therapeutic effect of the myoblasts invivo, we cultured a monolayer of C2C12 myoblasts on thermosensitive hydrogels, which was then transferred to the hindlimb ischemia tissue of athymic mice directly from the hydrogel by conformal contact. The transferred myoblast layer was retained for a longer period of time than an intramuscularly injected cell suspension. In addition, the morphology of the mice and laser Doppler perfusion (28 days after treatment) supported that the myoblast layer enhanced the therapeutic effects on the ischemic tissue. In summary, the transplantation of the C2C12 myoblast layer using a tissue transfer printing method could represent a new approach for the treatment of PAD by therapeutic angiogenesis.

Original languageEnglish
Pages (from-to)8258-8268
Number of pages11
JournalBiomaterials
Volume34
Issue number33
DOIs
Publication statusPublished - 2013 Nov 1

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Kim, D. W., Jun, I., Lee, T. J., Lee, J. H., Lee, Y. J., Jang, H. K., Kang, S., Park, K. D., Cho, S. W., Kim, B. S., & Shin, H. (2013). Therapeutic angiogenesis by a myoblast layer harvested by tissue transfer printing from cell-adhesive, thermosensitive hydrogels. Biomaterials, 34(33), 8258-8268. https://doi.org/10.1016/j.biomaterials.2013.07.071