Therapeutic effect of Active Hexose-Correlated Compound (AHCC) combined with CpG-ODN (oligodeoxynucleotide) in B16 melanoma murine model

Rosa Mistica Ignacio, Cheol Su Kim, Young Do Kim, Hak Min Lee, Xu Feng Qi, Soo-Ki Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

While Active Hexose Correlated Compound (AHCC) and CpG oligodeoxynucleotide (ODN) are separately known to modulate oxidative stress and immune responses in cancer patients, the combined effect of these two compounds is unknown. To clarify this, we investigated whether AHCC plus KSK-CpG ODN would be therapeutic in B16 melanoma mouse model, if so, and how in reduction-oxidation (redox) balance and cytokines network. We found that treatment groups (AHCC only, KSK-CpG ODN only and AHCC/KSK-CpG ODN) markedly reduced (p< 0.001) tumor size when compared to the positive control (PC) group. The total white blood cell (WBC) of AHCC only and KSK-CpG ODN only-treated groups showed significant lower counts than that of PC group. Next, the production of nitric oxide (NO) was significantly increased (p< 0.01) in AHCC/KSK-CpG ODN group compared to the PC group. Further, the redox balance was improved in AHCC/KSK-CpG ODN group through significantly low (p< 0.001) reactive oxygen species (ROS) production and significantly high (p< 0.05) glutathione peroxidase (GPx) activity compared to the PC group. Finally, AHCC/KSK-CpG ODN (p< 0.01) and KSK-CpG ODN (p< 0.001)-treated groups augmented tumor immune surveillance as shown by significantly increased level of anti-inflammatory cytokine (IL-10) and significantly decreased (p< 0.05) level of pro-tumorigenic IL-6 of AHCC/KSK-CpG ODN treated group as compared to the PC group. Collectively, our study indicates therapeutic effect of Active Hexose-Correlated Compound (AHCC) combined with KSK-CpG ODN in B16 melanoma murine model via balancing redox and cytokines network.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalCytokine
Volume76
Issue number2
DOIs
Publication statusPublished - 2015 Dec 1

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Experimental Melanomas
Oligodeoxyribonucleotides
Therapeutic Uses
Control Groups
Cytokines
Oxidation
Tumors
Active Hexose Correlated Compound
Neoplasms
Oxidative stress
Glutathione Peroxidase
Interleukin-10
Interleukin-6
Reactive Oxygen Species
Nitric Oxide
Oxidative Stress
Leukocytes
Blood
Anti-Inflammatory Agents
Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Ignacio, Rosa Mistica ; Kim, Cheol Su ; Kim, Young Do ; Lee, Hak Min ; Qi, Xu Feng ; Kim, Soo-Ki. / Therapeutic effect of Active Hexose-Correlated Compound (AHCC) combined with CpG-ODN (oligodeoxynucleotide) in B16 melanoma murine model. In: Cytokine. 2015 ; Vol. 76, No. 2. pp. 131-137.
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abstract = "While Active Hexose Correlated Compound (AHCC) and CpG oligodeoxynucleotide (ODN) are separately known to modulate oxidative stress and immune responses in cancer patients, the combined effect of these two compounds is unknown. To clarify this, we investigated whether AHCC plus KSK-CpG ODN would be therapeutic in B16 melanoma mouse model, if so, and how in reduction-oxidation (redox) balance and cytokines network. We found that treatment groups (AHCC only, KSK-CpG ODN only and AHCC/KSK-CpG ODN) markedly reduced (p< 0.001) tumor size when compared to the positive control (PC) group. The total white blood cell (WBC) of AHCC only and KSK-CpG ODN only-treated groups showed significant lower counts than that of PC group. Next, the production of nitric oxide (NO) was significantly increased (p< 0.01) in AHCC/KSK-CpG ODN group compared to the PC group. Further, the redox balance was improved in AHCC/KSK-CpG ODN group through significantly low (p< 0.001) reactive oxygen species (ROS) production and significantly high (p< 0.05) glutathione peroxidase (GPx) activity compared to the PC group. Finally, AHCC/KSK-CpG ODN (p< 0.01) and KSK-CpG ODN (p< 0.001)-treated groups augmented tumor immune surveillance as shown by significantly increased level of anti-inflammatory cytokine (IL-10) and significantly decreased (p< 0.05) level of pro-tumorigenic IL-6 of AHCC/KSK-CpG ODN treated group as compared to the PC group. Collectively, our study indicates therapeutic effect of Active Hexose-Correlated Compound (AHCC) combined with KSK-CpG ODN in B16 melanoma murine model via balancing redox and cytokines network.",
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Therapeutic effect of Active Hexose-Correlated Compound (AHCC) combined with CpG-ODN (oligodeoxynucleotide) in B16 melanoma murine model. / Ignacio, Rosa Mistica; Kim, Cheol Su; Kim, Young Do; Lee, Hak Min; Qi, Xu Feng; Kim, Soo-Ki.

In: Cytokine, Vol. 76, No. 2, 01.12.2015, p. 131-137.

Research output: Contribution to journalArticle

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AU - Ignacio, Rosa Mistica

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AU - Qi, Xu Feng

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