Therapeutic effect of resveratrol on oxidative stress in Graves’ orbitopathy orbital fibroblasts

Chang Yeom Kim, Hyun Jung Lee, Min Kyung Chae, Jung Woo Byun, Eun Jig Lee, Jin Sook Yoon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose. Multiple causative factors complicate the pathogenesis in Graves’ orbitopathy (GO). It has been suggested that oxidative stress contributes to the development and progression of GO. Therefore, we investigated the therapeutic effect of resveratrol, a potent antioxidant, upon oxidative stress levels in GO orbital fibroblasts in vitro. METHODS. Orbital fibroblasts were cultured from orbital connective tissues obtained from GO patients. Intracellular reactive oxygen species (ROS) levels and the expression of heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and thioredoxin (Trx), were measured after resveratrol treatment. Adipogenesis was induced, and ROS levels were examined during adipogenic differentiation. Western blot assay was performed to evaluate the effects of resveratrol on the expression of antioxidants levels and transcriptional regulators. Results. Treatment with 30 or 50 μM resveratrol reduced ROS production and HO-1 level induced by oxidative stress. Levels of Cu/Zn-SOD, catalase, and Trx were also reduced, while Mn-SOD increased with 50 μM resveratrol treatment. Resveratrol suppressed adipogenesis, reducing the number of adipocytes and suppressing the accumulation of lipid droplets. Treatment with 50 μM resveratrol also decreased ROS levels during adipogenesis. Expression of the transcriptional regulators phosphor–extracellular signal-regulated kinase and phospho– c-Jun NH(2)-terminal kinase significantly increased after treatment with 50 μM resveratrol, and decreased in response to inhibitors of each protein. Phosphonuclear factor kappa-lightchain- enhancer of activated B cells p65 levels also increased after treatment with 50 μM resveratrol. Conclusions. Resveratrol reduced ROS levels and inhibited adipogenesis in GO orbital fibroblasts in vitro. This study supports the potential use of resveratrol in GO treatment.

Original languageEnglish
Pages (from-to)6352-6361
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number11
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Therapeutic Uses
Oxidative Stress
Fibroblasts
Adipogenesis
Reactive Oxygen Species
Thioredoxins
Heme Oxygenase-1
Catalase
Therapeutics
Superoxide Dismutase
resveratrol
Phosphotransferases
Antioxidants
Adipocytes
Connective Tissue
B-Lymphocytes
Western Blotting

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

@article{3fde163cfaee463890ed2d29e6410cec,
title = "Therapeutic effect of resveratrol on oxidative stress in Graves’ orbitopathy orbital fibroblasts",
abstract = "Purpose. Multiple causative factors complicate the pathogenesis in Graves’ orbitopathy (GO). It has been suggested that oxidative stress contributes to the development and progression of GO. Therefore, we investigated the therapeutic effect of resveratrol, a potent antioxidant, upon oxidative stress levels in GO orbital fibroblasts in vitro. METHODS. Orbital fibroblasts were cultured from orbital connective tissues obtained from GO patients. Intracellular reactive oxygen species (ROS) levels and the expression of heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and thioredoxin (Trx), were measured after resveratrol treatment. Adipogenesis was induced, and ROS levels were examined during adipogenic differentiation. Western blot assay was performed to evaluate the effects of resveratrol on the expression of antioxidants levels and transcriptional regulators. Results. Treatment with 30 or 50 μM resveratrol reduced ROS production and HO-1 level induced by oxidative stress. Levels of Cu/Zn-SOD, catalase, and Trx were also reduced, while Mn-SOD increased with 50 μM resveratrol treatment. Resveratrol suppressed adipogenesis, reducing the number of adipocytes and suppressing the accumulation of lipid droplets. Treatment with 50 μM resveratrol also decreased ROS levels during adipogenesis. Expression of the transcriptional regulators phosphor–extracellular signal-regulated kinase and phospho– c-Jun NH(2)-terminal kinase significantly increased after treatment with 50 μM resveratrol, and decreased in response to inhibitors of each protein. Phosphonuclear factor kappa-lightchain- enhancer of activated B cells p65 levels also increased after treatment with 50 μM resveratrol. Conclusions. Resveratrol reduced ROS levels and inhibited adipogenesis in GO orbital fibroblasts in vitro. This study supports the potential use of resveratrol in GO treatment.",
author = "Kim, {Chang Yeom} and Lee, {Hyun Jung} and Chae, {Min Kyung} and Byun, {Jung Woo} and Lee, {Eun Jig} and Yoon, {Jin Sook}",
year = "2015",
month = "1",
day = "1",
doi = "10.1167/iovs.15-16870",
language = "English",
volume = "56",
pages = "6352--6361",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "11",

}

Therapeutic effect of resveratrol on oxidative stress in Graves’ orbitopathy orbital fibroblasts. / Kim, Chang Yeom; Lee, Hyun Jung; Chae, Min Kyung; Byun, Jung Woo; Lee, Eun Jig; Yoon, Jin Sook.

In: Investigative Ophthalmology and Visual Science, Vol. 56, No. 11, 01.01.2015, p. 6352-6361.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Therapeutic effect of resveratrol on oxidative stress in Graves’ orbitopathy orbital fibroblasts

AU - Kim, Chang Yeom

AU - Lee, Hyun Jung

AU - Chae, Min Kyung

AU - Byun, Jung Woo

AU - Lee, Eun Jig

AU - Yoon, Jin Sook

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Purpose. Multiple causative factors complicate the pathogenesis in Graves’ orbitopathy (GO). It has been suggested that oxidative stress contributes to the development and progression of GO. Therefore, we investigated the therapeutic effect of resveratrol, a potent antioxidant, upon oxidative stress levels in GO orbital fibroblasts in vitro. METHODS. Orbital fibroblasts were cultured from orbital connective tissues obtained from GO patients. Intracellular reactive oxygen species (ROS) levels and the expression of heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and thioredoxin (Trx), were measured after resveratrol treatment. Adipogenesis was induced, and ROS levels were examined during adipogenic differentiation. Western blot assay was performed to evaluate the effects of resveratrol on the expression of antioxidants levels and transcriptional regulators. Results. Treatment with 30 or 50 μM resveratrol reduced ROS production and HO-1 level induced by oxidative stress. Levels of Cu/Zn-SOD, catalase, and Trx were also reduced, while Mn-SOD increased with 50 μM resveratrol treatment. Resveratrol suppressed adipogenesis, reducing the number of adipocytes and suppressing the accumulation of lipid droplets. Treatment with 50 μM resveratrol also decreased ROS levels during adipogenesis. Expression of the transcriptional regulators phosphor–extracellular signal-regulated kinase and phospho– c-Jun NH(2)-terminal kinase significantly increased after treatment with 50 μM resveratrol, and decreased in response to inhibitors of each protein. Phosphonuclear factor kappa-lightchain- enhancer of activated B cells p65 levels also increased after treatment with 50 μM resveratrol. Conclusions. Resveratrol reduced ROS levels and inhibited adipogenesis in GO orbital fibroblasts in vitro. This study supports the potential use of resveratrol in GO treatment.

AB - Purpose. Multiple causative factors complicate the pathogenesis in Graves’ orbitopathy (GO). It has been suggested that oxidative stress contributes to the development and progression of GO. Therefore, we investigated the therapeutic effect of resveratrol, a potent antioxidant, upon oxidative stress levels in GO orbital fibroblasts in vitro. METHODS. Orbital fibroblasts were cultured from orbital connective tissues obtained from GO patients. Intracellular reactive oxygen species (ROS) levels and the expression of heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and thioredoxin (Trx), were measured after resveratrol treatment. Adipogenesis was induced, and ROS levels were examined during adipogenic differentiation. Western blot assay was performed to evaluate the effects of resveratrol on the expression of antioxidants levels and transcriptional regulators. Results. Treatment with 30 or 50 μM resveratrol reduced ROS production and HO-1 level induced by oxidative stress. Levels of Cu/Zn-SOD, catalase, and Trx were also reduced, while Mn-SOD increased with 50 μM resveratrol treatment. Resveratrol suppressed adipogenesis, reducing the number of adipocytes and suppressing the accumulation of lipid droplets. Treatment with 50 μM resveratrol also decreased ROS levels during adipogenesis. Expression of the transcriptional regulators phosphor–extracellular signal-regulated kinase and phospho– c-Jun NH(2)-terminal kinase significantly increased after treatment with 50 μM resveratrol, and decreased in response to inhibitors of each protein. Phosphonuclear factor kappa-lightchain- enhancer of activated B cells p65 levels also increased after treatment with 50 μM resveratrol. Conclusions. Resveratrol reduced ROS levels and inhibited adipogenesis in GO orbital fibroblasts in vitro. This study supports the potential use of resveratrol in GO treatment.

UR - http://www.scopus.com/inward/record.url?scp=84943563988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943563988&partnerID=8YFLogxK

U2 - 10.1167/iovs.15-16870

DO - 10.1167/iovs.15-16870

M3 - Article

C2 - 26436888

AN - SCOPUS:84943563988

VL - 56

SP - 6352

EP - 6361

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 11

ER -