Therapeutic effects of Holmium-166 chitosan complex in rat brain tumor model

Ryoong Huh, Yong Sook Park, Jong Doo Lee, Young Sun Chung, Yong Gou Park, Sang Sup Chung, JinWoo Chang

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Abstract

This study examined the effectiveness of Holmium-166 (Ho-166) chitosan complex therapy for a malignant glioma. Cultured C6 glioma cells (100,000 in 5 μl) were injected into the caudate/putamen of 200 - 250 gram Wistar rats. Five days later, a Ho-166 chitosan complex was injected into the same site of the glioma injection. Four injection doses were administered: the control group received PBS 10 μl, group 1 received an injection of 100 μ Ci (10 μl), group 2 received an injection of 50 μ Ci (5 μl), and group 3 received an injection of 10 μ Ci (1 μl). The average tumor volume for each group was 1.385 mm3 for the control group, 0.036 mm3 for group 1, 0.104 mm3 for group 2, and 0.111 mm3 for group 3. Compared with the control group, the size of the tumors in groups 1, 2 and 3 was reduced by an average of 97.4%, 92.5% and 91.9%, respectively. The Kaplan-Meier survival curve of group 2 was the longest, followed by groups 3, group 1 and the control. The mean survival was 22.8, 59, 60, and 44.6 days for the control group and groups 3, 2 and 1, respectively. H-E staining revealed that group 2 yielded the best results in the destruction of the malignant glioma. TUNEL staining and immunohistochemical studies indicated apoptotic features. The Ho-166 chitosan complex proved to be effective in destroying the malignant glioma.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalYonsei medical journal
Volume46
Issue number1
DOIs
Publication statusPublished - 2005 Feb 28

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Therapeutic Uses
Brain Neoplasms
Glioma
Control Groups
Injections
Staining and Labeling
Putamen
In Situ Nick-End Labeling
Kaplan-Meier Estimate
Tumor Burden
Wistar Rats
(166)holmium-chitosan complex
Neoplasms

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Huh, Ryoong ; Park, Yong Sook ; Lee, Jong Doo ; Chung, Young Sun ; Park, Yong Gou ; Chung, Sang Sup ; Chang, JinWoo. / Therapeutic effects of Holmium-166 chitosan complex in rat brain tumor model. In: Yonsei medical journal. 2005 ; Vol. 46, No. 1. pp. 51-60.
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Therapeutic effects of Holmium-166 chitosan complex in rat brain tumor model. / Huh, Ryoong; Park, Yong Sook; Lee, Jong Doo; Chung, Young Sun; Park, Yong Gou; Chung, Sang Sup; Chang, JinWoo.

In: Yonsei medical journal, Vol. 46, No. 1, 28.02.2005, p. 51-60.

Research output: Contribution to journalArticle

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N2 - This study examined the effectiveness of Holmium-166 (Ho-166) chitosan complex therapy for a malignant glioma. Cultured C6 glioma cells (100,000 in 5 μl) were injected into the caudate/putamen of 200 - 250 gram Wistar rats. Five days later, a Ho-166 chitosan complex was injected into the same site of the glioma injection. Four injection doses were administered: the control group received PBS 10 μl, group 1 received an injection of 100 μ Ci (10 μl), group 2 received an injection of 50 μ Ci (5 μl), and group 3 received an injection of 10 μ Ci (1 μl). The average tumor volume for each group was 1.385 mm3 for the control group, 0.036 mm3 for group 1, 0.104 mm3 for group 2, and 0.111 mm3 for group 3. Compared with the control group, the size of the tumors in groups 1, 2 and 3 was reduced by an average of 97.4%, 92.5% and 91.9%, respectively. The Kaplan-Meier survival curve of group 2 was the longest, followed by groups 3, group 1 and the control. The mean survival was 22.8, 59, 60, and 44.6 days for the control group and groups 3, 2 and 1, respectively. H-E staining revealed that group 2 yielded the best results in the destruction of the malignant glioma. TUNEL staining and immunohistochemical studies indicated apoptotic features. The Ho-166 chitosan complex proved to be effective in destroying the malignant glioma.

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