Therapeutic effects of late outgrowth endothelial progenitor cells or mesenchymal stem cells derived from human umbilical cord blood on infarct repair

Sung Whan Kim, Hong Lian Jin, seokmin kang, Sinyoung Kim, Kyung Jong Yoo, Yangsoo Jang, Hyun Ok Kim, Youngsup Yoon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background This study sought to systematically investigate the derivation of late outgrowth endothelial progenitor cells (late EPC) and mesenchymal stem cells (MSC) from umbilical cord blood (UCB) and to examine their therapeutic effects on myocardial infarction (MI). Methods The expression of angiogenic genes was determined by qRT-PCR. Myocardial infarction (MI) was induced in rats, and cells were directly transplanted into the border regions of ischemic heart tissue. Results Culture of UCB mononuclear cells yielded two distinct types of cells by morphology after 2 weeks in the same culture conditions. These cells were identified as late EPC and MSC, and each was intramyocardially injected into rat hearts after induction of MI. Echocardiography and histologic analyses demonstrated that both EPC and MSC improved cardiac function and enhanced vascularization, although fibrosis was reduced only in the EPC transplanted hearts. Different paracrine factors were enriched in EPC and MSC. However, once injected into the hearts, they induced similar types of paracrine factors in the heart. Transplanted EPC or MSC were mostly localized at the perivascular areas. This study demonstrated that EPC and MSC can be simultaneously derived from UCB under the same initial culture conditions, and that common paracrine factors are involved in the repair of MI. Conclusion Late EPC and MSC are effective for infarct repair, apparently mediated through common humoral mechanisms.

Original languageEnglish
Pages (from-to)498-507
Number of pages10
JournalInternational Journal of Cardiology
Volume203
DOIs
Publication statusPublished - 2016 Jan 15

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Therapeutic Uses
Mesenchymal Stromal Cells
Fetal Blood
Myocardial Infarction
Endothelial Progenitor Cells
Echocardiography
erucylphosphocholine
Blood Cells
Fibrosis
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Therapeutic effects of late outgrowth endothelial progenitor cells or mesenchymal stem cells derived from human umbilical cord blood on infarct repair",
abstract = "Background This study sought to systematically investigate the derivation of late outgrowth endothelial progenitor cells (late EPC) and mesenchymal stem cells (MSC) from umbilical cord blood (UCB) and to examine their therapeutic effects on myocardial infarction (MI). Methods The expression of angiogenic genes was determined by qRT-PCR. Myocardial infarction (MI) was induced in rats, and cells were directly transplanted into the border regions of ischemic heart tissue. Results Culture of UCB mononuclear cells yielded two distinct types of cells by morphology after 2 weeks in the same culture conditions. These cells were identified as late EPC and MSC, and each was intramyocardially injected into rat hearts after induction of MI. Echocardiography and histologic analyses demonstrated that both EPC and MSC improved cardiac function and enhanced vascularization, although fibrosis was reduced only in the EPC transplanted hearts. Different paracrine factors were enriched in EPC and MSC. However, once injected into the hearts, they induced similar types of paracrine factors in the heart. Transplanted EPC or MSC were mostly localized at the perivascular areas. This study demonstrated that EPC and MSC can be simultaneously derived from UCB under the same initial culture conditions, and that common paracrine factors are involved in the repair of MI. Conclusion Late EPC and MSC are effective for infarct repair, apparently mediated through common humoral mechanisms.",
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Therapeutic effects of late outgrowth endothelial progenitor cells or mesenchymal stem cells derived from human umbilical cord blood on infarct repair. / Kim, Sung Whan; Jin, Hong Lian; kang, seokmin; Kim, Sinyoung; Yoo, Kyung Jong; Jang, Yangsoo; Kim, Hyun Ok; Yoon, Youngsup.

In: International Journal of Cardiology, Vol. 203, 15.01.2016, p. 498-507.

Research output: Contribution to journalArticle

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AU - Kim, Sung Whan

AU - Jin, Hong Lian

AU - kang, seokmin

AU - Kim, Sinyoung

AU - Yoo, Kyung Jong

AU - Jang, Yangsoo

AU - Kim, Hyun Ok

AU - Yoon, Youngsup

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