Therapeutic effects of water soluble Danshen extracts on atherosclerosis

Yoon Hee Cho, Cheol Ryong Ku, Zhen Yu Hong, Ji Hoe Heo, Eun Hee Kim, Dong Hoon Choi, Dongkyu Kim, Ae Jung Kim, Cheol Soon Lee, Mankil Jung, Hyun Chul Lee, Miran Seo, Eun Jig Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Danshen is a traditional Chinese medicine with many beneficial effects on cardiovascular diseases. The aim of this study was to evaluate the mechanisms responsible for the antiatherogenic effect of water soluble Danshen extracts (DEs). Rat vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs) were treated with DE. To evaluate the effects of DE in vivo, carotid balloon injury and tail vein thrombosis were induced in Sprague-Dawley (SD) rats and iliac artery stent was induced in New Zealand white rabbits. The inhibitory action of DE on platelet aggregation was confirmed with an impedance aggregometer. DE inhibited the production of reactive oxygen species, and the migration and proliferation of platelet-derived growth factor-BB stimulated VSMCs. Furthermore, DE prevented inflammation and apoptosis in HUVECs. Both effects of DE were reconfirmed in both rat models. DE treatment attenuated platelet aggregation in both in vivo and ex vivo conditions. Pretreatment with DE prevented tail vein thrombosis, which is normally induced by -carrageenan injection. Lastly, DE-treated rabbits showed decreased in-stent restenosis of stented iliac arteries. These results suggest that water soluble DE modulates key atherogenic events in VSMCs, endothelial cells, and platelets in both in vitro and in vivo conditions.

Original languageEnglish
Article number623639
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
Publication statusPublished - 2013 Feb 11

Fingerprint

Therapeutic Uses
Atherosclerosis
Water
Vascular Smooth Muscle
Smooth Muscle Myocytes
Iliac Artery
Human Umbilical Vein Endothelial Cells
Platelet Aggregation
Stents
Tail
Veins
Thrombosis
dan-shen root extract
Salvia miltiorrhiza
Rabbits
Carrageenan
Chinese Traditional Medicine
Electric Impedance
Sprague Dawley Rats
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Cho, Yoon Hee ; Ku, Cheol Ryong ; Hong, Zhen Yu ; Heo, Ji Hoe ; Kim, Eun Hee ; Choi, Dong Hoon ; Kim, Dongkyu ; Kim, Ae Jung ; Lee, Cheol Soon ; Jung, Mankil ; Lee, Hyun Chul ; Seo, Miran ; Lee, Eun Jig. / Therapeutic effects of water soluble Danshen extracts on atherosclerosis. In: Evidence-based Complementary and Alternative Medicine. 2013 ; Vol. 2013.
@article{a5179f0dff5841e2a077fad2771d171f,
title = "Therapeutic effects of water soluble Danshen extracts on atherosclerosis",
abstract = "Danshen is a traditional Chinese medicine with many beneficial effects on cardiovascular diseases. The aim of this study was to evaluate the mechanisms responsible for the antiatherogenic effect of water soluble Danshen extracts (DEs). Rat vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs) were treated with DE. To evaluate the effects of DE in vivo, carotid balloon injury and tail vein thrombosis were induced in Sprague-Dawley (SD) rats and iliac artery stent was induced in New Zealand white rabbits. The inhibitory action of DE on platelet aggregation was confirmed with an impedance aggregometer. DE inhibited the production of reactive oxygen species, and the migration and proliferation of platelet-derived growth factor-BB stimulated VSMCs. Furthermore, DE prevented inflammation and apoptosis in HUVECs. Both effects of DE were reconfirmed in both rat models. DE treatment attenuated platelet aggregation in both in vivo and ex vivo conditions. Pretreatment with DE prevented tail vein thrombosis, which is normally induced by -carrageenan injection. Lastly, DE-treated rabbits showed decreased in-stent restenosis of stented iliac arteries. These results suggest that water soluble DE modulates key atherogenic events in VSMCs, endothelial cells, and platelets in both in vitro and in vivo conditions.",
author = "Cho, {Yoon Hee} and Ku, {Cheol Ryong} and Hong, {Zhen Yu} and Heo, {Ji Hoe} and Kim, {Eun Hee} and Choi, {Dong Hoon} and Dongkyu Kim and Kim, {Ae Jung} and Lee, {Cheol Soon} and Mankil Jung and Lee, {Hyun Chul} and Miran Seo and Lee, {Eun Jig}",
year = "2013",
month = "2",
day = "11",
doi = "10.1155/2013/623639",
language = "English",
volume = "2013",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",

}

Therapeutic effects of water soluble Danshen extracts on atherosclerosis. / Cho, Yoon Hee; Ku, Cheol Ryong; Hong, Zhen Yu; Heo, Ji Hoe; Kim, Eun Hee; Choi, Dong Hoon; Kim, Dongkyu; Kim, Ae Jung; Lee, Cheol Soon; Jung, Mankil; Lee, Hyun Chul; Seo, Miran; Lee, Eun Jig.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 623639, 11.02.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Therapeutic effects of water soluble Danshen extracts on atherosclerosis

AU - Cho, Yoon Hee

AU - Ku, Cheol Ryong

AU - Hong, Zhen Yu

AU - Heo, Ji Hoe

AU - Kim, Eun Hee

AU - Choi, Dong Hoon

AU - Kim, Dongkyu

AU - Kim, Ae Jung

AU - Lee, Cheol Soon

AU - Jung, Mankil

AU - Lee, Hyun Chul

AU - Seo, Miran

AU - Lee, Eun Jig

PY - 2013/2/11

Y1 - 2013/2/11

N2 - Danshen is a traditional Chinese medicine with many beneficial effects on cardiovascular diseases. The aim of this study was to evaluate the mechanisms responsible for the antiatherogenic effect of water soluble Danshen extracts (DEs). Rat vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs) were treated with DE. To evaluate the effects of DE in vivo, carotid balloon injury and tail vein thrombosis were induced in Sprague-Dawley (SD) rats and iliac artery stent was induced in New Zealand white rabbits. The inhibitory action of DE on platelet aggregation was confirmed with an impedance aggregometer. DE inhibited the production of reactive oxygen species, and the migration and proliferation of platelet-derived growth factor-BB stimulated VSMCs. Furthermore, DE prevented inflammation and apoptosis in HUVECs. Both effects of DE were reconfirmed in both rat models. DE treatment attenuated platelet aggregation in both in vivo and ex vivo conditions. Pretreatment with DE prevented tail vein thrombosis, which is normally induced by -carrageenan injection. Lastly, DE-treated rabbits showed decreased in-stent restenosis of stented iliac arteries. These results suggest that water soluble DE modulates key atherogenic events in VSMCs, endothelial cells, and platelets in both in vitro and in vivo conditions.

AB - Danshen is a traditional Chinese medicine with many beneficial effects on cardiovascular diseases. The aim of this study was to evaluate the mechanisms responsible for the antiatherogenic effect of water soluble Danshen extracts (DEs). Rat vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs) were treated with DE. To evaluate the effects of DE in vivo, carotid balloon injury and tail vein thrombosis were induced in Sprague-Dawley (SD) rats and iliac artery stent was induced in New Zealand white rabbits. The inhibitory action of DE on platelet aggregation was confirmed with an impedance aggregometer. DE inhibited the production of reactive oxygen species, and the migration and proliferation of platelet-derived growth factor-BB stimulated VSMCs. Furthermore, DE prevented inflammation and apoptosis in HUVECs. Both effects of DE were reconfirmed in both rat models. DE treatment attenuated platelet aggregation in both in vivo and ex vivo conditions. Pretreatment with DE prevented tail vein thrombosis, which is normally induced by -carrageenan injection. Lastly, DE-treated rabbits showed decreased in-stent restenosis of stented iliac arteries. These results suggest that water soluble DE modulates key atherogenic events in VSMCs, endothelial cells, and platelets in both in vitro and in vivo conditions.

UR - http://www.scopus.com/inward/record.url?scp=84873378251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873378251&partnerID=8YFLogxK

U2 - 10.1155/2013/623639

DO - 10.1155/2013/623639

M3 - Article

C2 - 23401716

AN - SCOPUS:84873378251

VL - 2013

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

M1 - 623639

ER -