Thermodynamics of DNA condensation induced by poly(ethylene glycol)- block -polylysine through polyion complex micelle formation

Wankee Kim, Yuichi Yamasaki, Woo Dong Jang, Kazunori Kataoka

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Isothermal titration calorimetry (ITC) was carried out to explore the condensation process of plasmid DNA (pDNA) molecules induced by poly(ethylene glycol)-poly(l-lysine) block copolymer (PEG-PLL) as a condensing agent. The ITC curves measured can be divided into two distinctive endothermic binding processes: the first was the binding of PEG-PLL to the elongated pDNA, and the second was the binding that accompanied the pDNA conformational transition. The thermodynamic parameters were obtained by fitting each ITC curve using our recently developed fitting method. The binding of PEG-PLL to the pDNA was accompanied by a small increase in enthalpy, a large increase in entropy, and a large decrease in free energy. The binding stabilized as the polymerization degree of PLL on PEG-PLL increased and the salt concentration decreased. Changes in the thermodynamic parameters are discussed in relation to both the polymerization degree of PLL on PEG-PLL and the salt concentration.

Original languageEnglish
Pages (from-to)1180-1186
Number of pages7
JournalBiomacromolecules
Volume11
Issue number5
DOIs
Publication statusPublished - 2010 May 10

Fingerprint

Polylysine
Micelles
Polyethylene glycols
Lysine
Block copolymers
Condensation
DNA
Thermodynamics
Plasmids
Calorimetry
Titration
Phase locked loops
Salts
Polymerization
Free energy
Enthalpy
Entropy
Molecules

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

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abstract = "Isothermal titration calorimetry (ITC) was carried out to explore the condensation process of plasmid DNA (pDNA) molecules induced by poly(ethylene glycol)-poly(l-lysine) block copolymer (PEG-PLL) as a condensing agent. The ITC curves measured can be divided into two distinctive endothermic binding processes: the first was the binding of PEG-PLL to the elongated pDNA, and the second was the binding that accompanied the pDNA conformational transition. The thermodynamic parameters were obtained by fitting each ITC curve using our recently developed fitting method. The binding of PEG-PLL to the pDNA was accompanied by a small increase in enthalpy, a large increase in entropy, and a large decrease in free energy. The binding stabilized as the polymerization degree of PLL on PEG-PLL increased and the salt concentration decreased. Changes in the thermodynamic parameters are discussed in relation to both the polymerization degree of PLL on PEG-PLL and the salt concentration.",
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Thermodynamics of DNA condensation induced by poly(ethylene glycol)- block -polylysine through polyion complex micelle formation. / Kim, Wankee; Yamasaki, Yuichi; Jang, Woo Dong; Kataoka, Kazunori.

In: Biomacromolecules, Vol. 11, No. 5, 10.05.2010, p. 1180-1186.

Research output: Contribution to journalArticle

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