Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens

Ga Yeon Lee, Ji Hong Bong, Ji Yun Kim, Gu Yoo, Min Park, Min Jung Kang, Joachim Jose, Jae Chul Pyun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The autoimmune diseases systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS) are diagnosed by detection of autoantibodies against Ro and La protein autoantigens, respectively. In this work, the diagnosis of autoimmune diseases SLE and SS was demonstrated using thermophoresis of Escherichia coli with the autodisplayed autoantigens. Ro and La protein autoantigens were autodisplayed by constitutive expression together with a fluorescent protein called tdTomato in the cytosol. The binding affinity of the autodisplayed autoantigens was tested against positive and control sera by using FACS as a reference method. The factors influencing interactions between E. coli with autodisplayed autoantigens and autoantibodies in sera during thermophoresis were analyzed by measurement of cell surface charge and size before and after interaction. Finally, the thermophoretic diagnosis of autoimmune diseases SLE and SS was demonstrated using sera from patients afflicted with the respective diseases by estimating sensitivity and selectivity from ROC plots.

Original languageEnglish
Pages (from-to)106-114
Number of pages9
JournalAnalytica Chimica Acta
Volume1055
DOIs
Publication statusPublished - 2019 May 9

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Fingerprint Dive into the research topics of 'Thermophoretic diagnosis of autoimmune diseases based on Escherichia coli with autodisplayed autoantigens'. Together they form a unique fingerprint.

  • Cite this