Immunoassays are used to detect a target analyte by using specific interaction with detection antibodies which form antigen-antibody complexes. In thermophoretic immunoassays the antigen-antibody complexes make slow thermophoresis (movement by thermal stimulation) in comparison with unbound antigens and antibodies. In this work, a sensitive thermophoretic immunoassay was demonstrated by mixing outer membrane (OM) particles of E. coli with autodisplayed Z-domains. In order to compare the influence of OM particles with autodisplayed Z-domains on the sensitivity of thermophoretic immunoassay, the conventional method only with target analyte and detection antibodies was compared with the hyper sensitive thermophoretic immunoassay by mixing OM particles with autodisplayed Z-domains, and the affinity constant was calculated. Finally, the diagnosis of inflammation by detection of a biomarker called C-reactive protein (CRP) was carried out by demonstrating the thermophoretic immunoassay for CRP based on autodisplayedZ-domains could be applied for the medical diagnosis.
Bibliographical noteFunding Information:
We are very grateful to K. Langer, Pharm. Tech., WWU Münster for the opportunity to do the photon correlation spectroscopy experiments in his lab. This work was supported by the Nano-Convergence Foundation [grant number: R201602210 ] funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea) and the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the Industry Technology Development Program [grant number: 10063335 ] funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea) , and by the National Research Foundation of Korea [grant number: NRF-2017R1A6A3A11034081, NRF-2017R1A2B4004077 , NRF-2017R1A2B2004398 , NRF-2016R1D1A3B03934169 ], and by the Yonsei University Research Fund(Yonsei Frontier Lab. Young Researcher Supporting Program) of 2017, and by the Hallym University Research Fund , 2017 (HRF-201709-014).
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry