Thick airway surface liquid volume and weak mucin expression in pendrin-deficient human airway epithelia

Hyun Jae Lee, Jee Eun Yoo, Wan Namkung, Hyung Ju Cho, Kyubo Kim, Joo Wan Kang, Joo Heon Yoon, Jae Young Choi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pendrin is an anion exchanger whose mutations are known to cause hearing loss. However, recent data support the linkage between pendrin expression and airway diseases, such as asthma. To evaluate the role of pendrin in the regulation of the airway surface liquid (ASL) volume and mucin expression, we investigated the function and expression of pendrin and ion channels and anion exchangers. Human nasal epithelial cells were cultured from 16 deaf patients carrying pendrin mutations (DFNB4) and 17 controls. The cells were treated with IL-13 to induce mucus hypersecretion. Airway surface liquid thickness was measured and real-time polymerase chain reaction was performed targeting various transporters and MUC5AC. Anion exchanger activity was measured using a pH-sensitive fluorescent probe. Periodic acid-Schiff staining was performed on the cultured cells and inferior turbinate tissues. The ASL layer of the nasal epithelia from DFNB4 subjects was thicker than the controls, and the difference became more prominent following IL-13 stimulation. There was no difference in anion exchange activity after IL-13 treatment in the cells from DFNB4 patients, while it increased in the controls. Goblet cell metaplasia induced by IL-13 treatment seen in the controls was not observed in the DFNB4 cells. Furthermore, the periodic acid-Schiff staining-positive area was lesser in the inferior turbinate tissues from DFNB4 patients that those from controls. Pendrin plays a critical role in ASL volume regulation and mucin expression as pendrin-deficient airway epithelial cells are refractory to stimulation with IL-13. Specific blockers targeting pendrin in the airways may therefore have therapeutic potential in the treatment of allergic airway diseases.

Original languageEnglish
Article numbere12480
JournalPhysiological Reports
Volume3
Issue number8
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Interleukin-13
Mucins
Epithelium
Anions
Turbinates
Periodic Acid
Epithelial Cells
Staining and Labeling
Mutation
Goblet Cells
Nasal Mucosa
Information Storage and Retrieval
Metaplasia
Mucus
Therapeutics
Ion Channels
Fluorescent Dyes
Hearing Loss
Nose
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Lee, Hyun Jae ; Yoo, Jee Eun ; Namkung, Wan ; Cho, Hyung Ju ; Kim, Kyubo ; Kang, Joo Wan ; Yoon, Joo Heon ; Choi, Jae Young. / Thick airway surface liquid volume and weak mucin expression in pendrin-deficient human airway epithelia. In: Physiological Reports. 2015 ; Vol. 3, No. 8.
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abstract = "Pendrin is an anion exchanger whose mutations are known to cause hearing loss. However, recent data support the linkage between pendrin expression and airway diseases, such as asthma. To evaluate the role of pendrin in the regulation of the airway surface liquid (ASL) volume and mucin expression, we investigated the function and expression of pendrin and ion channels and anion exchangers. Human nasal epithelial cells were cultured from 16 deaf patients carrying pendrin mutations (DFNB4) and 17 controls. The cells were treated with IL-13 to induce mucus hypersecretion. Airway surface liquid thickness was measured and real-time polymerase chain reaction was performed targeting various transporters and MUC5AC. Anion exchanger activity was measured using a pH-sensitive fluorescent probe. Periodic acid-Schiff staining was performed on the cultured cells and inferior turbinate tissues. The ASL layer of the nasal epithelia from DFNB4 subjects was thicker than the controls, and the difference became more prominent following IL-13 stimulation. There was no difference in anion exchange activity after IL-13 treatment in the cells from DFNB4 patients, while it increased in the controls. Goblet cell metaplasia induced by IL-13 treatment seen in the controls was not observed in the DFNB4 cells. Furthermore, the periodic acid-Schiff staining-positive area was lesser in the inferior turbinate tissues from DFNB4 patients that those from controls. Pendrin plays a critical role in ASL volume regulation and mucin expression as pendrin-deficient airway epithelial cells are refractory to stimulation with IL-13. Specific blockers targeting pendrin in the airways may therefore have therapeutic potential in the treatment of allergic airway diseases.",
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Thick airway surface liquid volume and weak mucin expression in pendrin-deficient human airway epithelia. / Lee, Hyun Jae; Yoo, Jee Eun; Namkung, Wan; Cho, Hyung Ju; Kim, Kyubo; Kang, Joo Wan; Yoon, Joo Heon; Choi, Jae Young.

In: Physiological Reports, Vol. 3, No. 8, e12480, 01.01.2015.

Research output: Contribution to journalArticle

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AU - Lee, Hyun Jae

AU - Yoo, Jee Eun

AU - Namkung, Wan

AU - Cho, Hyung Ju

AU - Kim, Kyubo

AU - Kang, Joo Wan

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AU - Choi, Jae Young

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AB - Pendrin is an anion exchanger whose mutations are known to cause hearing loss. However, recent data support the linkage between pendrin expression and airway diseases, such as asthma. To evaluate the role of pendrin in the regulation of the airway surface liquid (ASL) volume and mucin expression, we investigated the function and expression of pendrin and ion channels and anion exchangers. Human nasal epithelial cells were cultured from 16 deaf patients carrying pendrin mutations (DFNB4) and 17 controls. The cells were treated with IL-13 to induce mucus hypersecretion. Airway surface liquid thickness was measured and real-time polymerase chain reaction was performed targeting various transporters and MUC5AC. Anion exchanger activity was measured using a pH-sensitive fluorescent probe. Periodic acid-Schiff staining was performed on the cultured cells and inferior turbinate tissues. The ASL layer of the nasal epithelia from DFNB4 subjects was thicker than the controls, and the difference became more prominent following IL-13 stimulation. There was no difference in anion exchange activity after IL-13 treatment in the cells from DFNB4 patients, while it increased in the controls. Goblet cell metaplasia induced by IL-13 treatment seen in the controls was not observed in the DFNB4 cells. Furthermore, the periodic acid-Schiff staining-positive area was lesser in the inferior turbinate tissues from DFNB4 patients that those from controls. Pendrin plays a critical role in ASL volume regulation and mucin expression as pendrin-deficient airway epithelial cells are refractory to stimulation with IL-13. Specific blockers targeting pendrin in the airways may therefore have therapeutic potential in the treatment of allergic airway diseases.

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