Thin-film-based sensitivity enhancement for total internal reflection fluorescence live-cell imaging

Kyujung Kim, Eun Jin Cho, Yong Min Huh, Donghyun Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated experimentally the evanescent field enhancement based on dielectric thin films in total internal reflection microscopy. The sample employed two layers of Al2O3 and SiO2 deposited on an SF10 glass substrate. Field intensity enhancement measured by fluorescent excitation of microbeads relative to that of a control sample without dielectric films was polarization dependent, determined as 4.2 and 2.4 for TE and TM polarizations, respectively, and was in good agreement with numerical results. The thin-film-based field enhancement was also applied to live-cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively.

Original languageEnglish
Pages (from-to)3062-3064
Number of pages3
JournalOptics Letters
Volume32
Issue number21
DOIs
Publication statusPublished - 2007 Nov 1

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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