Three-Dimensional Electroconductive Hyaluronic Acid Hydrogels Incorporated with Carbon Nanotubes and Polypyrrole by Catechol-Mediated Dispersion Enhance Neurogenesis of Human Neural Stem Cells

Jisoo Shin, Eun Jung Choi, Jung Ho Cho, Ann Na Cho, Yoonhee Jin, Kisuk Yang, Changsik Song, Seung-Woo Cho

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Electrically conductive hyaluronic acid (HA) hydrogels incorporated with single-walled carbon nanotubes (CNTs) and/or polypyrrole (PPy) were developed to promote differentiation of human neural stem/progenitor cells (hNSPCs). The CNT and PPy nanocomposites, which do not easily disperse in aqueous phases, dispersed well and were efficiently incorporated into catechol-functionalized HA (HA-CA) hydrogels by the oxidative catechol chemistry used for hydrogel cross-linking. The prepared electroconductive HA hydrogels provided dynamic, electrically conductive three-dimensional (3D) extracellular matrix environments that were biocompatible with hNSPCs. The HA-CA hydrogels containing CNT and/or PPy significantly promoted neuronal differentiation of human fetal neural stem cells (hfNSCs) and human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) with improved electrophysiological functionality when compared to differentiation of these cells in a bare HA-CA hydrogel without electroconductive motifs. Calcium channel expression was upregulated, depolarization was activated, and intracellular calcium influx was increased in hNSPCs that were differentiated in 3D electroconductive HA-CA hydrogels; these data suggest a potential mechanism for stem cell neurogenesis. Overall, our bioinspired, electroconductive HA hydrogels provide a promising cell-culture platform and tissue-engineering scaffold to improve neuronal regeneration.

Original languageEnglish
Pages (from-to)3060-3072
Number of pages13
JournalBiomacromolecules
Volume18
Issue number10
DOIs
Publication statusPublished - 2017 Oct 9

Fingerprint

Hyaluronic acid
Carbon Nanotubes
Hydrogels
Polypyrroles
Hyaluronic Acid
Stem cells
Carbon nanotubes
Hydrogel
Calcium
Tissue Scaffolds
polypyrrole
catechol
Depolarization
Single-walled carbon nanotubes (SWCN)
Calcium Channels
Scaffolds (biology)
Tissue engineering
Cell culture
Nanocomposites
Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Shin, Jisoo ; Choi, Eun Jung ; Cho, Jung Ho ; Cho, Ann Na ; Jin, Yoonhee ; Yang, Kisuk ; Song, Changsik ; Cho, Seung-Woo. / Three-Dimensional Electroconductive Hyaluronic Acid Hydrogels Incorporated with Carbon Nanotubes and Polypyrrole by Catechol-Mediated Dispersion Enhance Neurogenesis of Human Neural Stem Cells. In: Biomacromolecules. 2017 ; Vol. 18, No. 10. pp. 3060-3072.
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abstract = "Electrically conductive hyaluronic acid (HA) hydrogels incorporated with single-walled carbon nanotubes (CNTs) and/or polypyrrole (PPy) were developed to promote differentiation of human neural stem/progenitor cells (hNSPCs). The CNT and PPy nanocomposites, which do not easily disperse in aqueous phases, dispersed well and were efficiently incorporated into catechol-functionalized HA (HA-CA) hydrogels by the oxidative catechol chemistry used for hydrogel cross-linking. The prepared electroconductive HA hydrogels provided dynamic, electrically conductive three-dimensional (3D) extracellular matrix environments that were biocompatible with hNSPCs. The HA-CA hydrogels containing CNT and/or PPy significantly promoted neuronal differentiation of human fetal neural stem cells (hfNSCs) and human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) with improved electrophysiological functionality when compared to differentiation of these cells in a bare HA-CA hydrogel without electroconductive motifs. Calcium channel expression was upregulated, depolarization was activated, and intracellular calcium influx was increased in hNSPCs that were differentiated in 3D electroconductive HA-CA hydrogels; these data suggest a potential mechanism for stem cell neurogenesis. Overall, our bioinspired, electroconductive HA hydrogels provide a promising cell-culture platform and tissue-engineering scaffold to improve neuronal regeneration.",
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Three-Dimensional Electroconductive Hyaluronic Acid Hydrogels Incorporated with Carbon Nanotubes and Polypyrrole by Catechol-Mediated Dispersion Enhance Neurogenesis of Human Neural Stem Cells. / Shin, Jisoo; Choi, Eun Jung; Cho, Jung Ho; Cho, Ann Na; Jin, Yoonhee; Yang, Kisuk; Song, Changsik; Cho, Seung-Woo.

In: Biomacromolecules, Vol. 18, No. 10, 09.10.2017, p. 3060-3072.

Research output: Contribution to journalArticle

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AU - Shin, Jisoo

AU - Choi, Eun Jung

AU - Cho, Jung Ho

AU - Cho, Ann Na

AU - Jin, Yoonhee

AU - Yang, Kisuk

AU - Song, Changsik

AU - Cho, Seung-Woo

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