Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device

Sewoon Han, Kisuk Yang, Yoojin Shin, Jung Seung Lee, Roger D. Kamm, Seok Chung, Seung Woo Cho

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.

Original languageEnglish
Pages (from-to)2305-2308
Number of pages4
JournalLab on a chip
Volume12
Issue number13
DOIs
Publication statusPublished - 2012 Jul 7

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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