Three novel cis-acting elements required for efficient plus-strand DNA synthesis of the hepatitis B virus genome

Jehan Lee, Myeong Kyun Shin, Hye Jin Lee, Gyesoon Yoon, Wang Shick Ryu

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Synthesis of the relaxed-circular (RC) DNA genomes of hepadnaviruses by reverse transcriptase involves two template switches during plus-strand DNA synthesis. These template switches require repeat sequences (so-called donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. To determine cis-acting elements apart from the donor and acceptor sites that are required for plus-strand RC DNA synthesis by hepatitis B virus (HBV), a series of mutants bearing a small deletion were made and analyzed for their impact on the viral genome synthesis. We found three novel cis-acting elements in the HBV genome: one element, located in the middle of the minus strand, is indispensable, whereas the other two elements, located near either end of the minus strand, contribute modestly to the plus-strand RC DNA synthesis. The data indicated that the first element facilitates plus-strand RNA primer translocation or subsequent elongation during plus-strand RC DNA synthesis, while the last two elements, although distantly located on the minus strand, act at multiple steps to promote plus-strand RC DNA synthesis. The necessity of multiple cis-acting elements on the minus-strand template reflects the complex nature of hepadnavirus reverse transcription.

Original languageEnglish
Pages (from-to)7455-7464
Number of pages10
JournalJournal of Virology
Volume78
Issue number14
DOIs
Publication statusPublished - 2004 Jul

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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