Three-photon light-sheet fluorescence microscopy

Adrià Escobet-Montalbán, Federico M. Gasparoli, Jonathan Nylk, Pengfei Liu, Zhengyi Yang, Kishan Dholakia

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Abstract

We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low-photodamage technique for fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime, enhancing its penetration depth. Our present study uses a conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 μm diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams.

Original languageEnglish
Pages (from-to)5484-5487
Number of pages4
JournalOptics Letters
Volume43
Issue number21
DOIs
Publication statusPublished - 2018 Nov 1

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All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Escobet-Montalbán, A., Gasparoli, F. M., Nylk, J., Liu, P., Yang, Z., & Dholakia, K. (2018). Three-photon light-sheet fluorescence microscopy. Optics Letters, 43(21), 5484-5487. https://doi.org/10.1364/OL.43.005484