Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis

Ji Yeon Noh, Jung U. Shin, Chang Ook Park, Nara Lee, Shan Jin, Seo Hyeong Kim, Ji Hye Kim, Arim Min, Myeong Heon Shin, Kwanghoon Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein L-plastin and confirmed upregulation of L-plastin and p-L- plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of L-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of L-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of L-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-L- plastin as a potential drug target for eosinophil-targeted allergy therapy.

Original languageEnglish
Pages (from-to)880-886
Number of pages7
JournalExperimental dermatology
Volume25
Issue number11
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

Phosphorylation
Atopic Dermatitis
Eosinophils
Cytokines
Allergies
Interleukin-13
thymic stromal lymphopoietin
plastin
Interleukin-3
Interleukin-5
Infiltration
Interleukin-4
Protein Kinase C
Assays
Proteomics
Skin
Proteins
Hypersensitivity
Up-Regulation
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Noh, Ji Yeon ; Shin, Jung U. ; Park, Chang Ook ; Lee, Nara ; Jin, Shan ; Kim, Seo Hyeong ; Kim, Ji Hye ; Min, Arim ; Shin, Myeong Heon ; Lee, Kwanghoon. / Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis. In: Experimental dermatology. 2016 ; Vol. 25, No. 11. pp. 880-886.
@article{e53005a229994b1390c2470734c9f01a,
title = "Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis",
abstract = "Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein L-plastin and confirmed upregulation of L-plastin and p-L- plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of L-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of L-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of L-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-L- plastin as a potential drug target for eosinophil-targeted allergy therapy.",
author = "Noh, {Ji Yeon} and Shin, {Jung U.} and Park, {Chang Ook} and Nara Lee and Shan Jin and Kim, {Seo Hyeong} and Kim, {Ji Hye} and Arim Min and Shin, {Myeong Heon} and Kwanghoon Lee",
year = "2016",
month = "11",
day = "1",
doi = "10.1111/exd.13111",
language = "English",
volume = "25",
pages = "880--886",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "11",

}

Noh, JY, Shin, JU, Park, CO, Lee, N, Jin, S, Kim, SH, Kim, JH, Min, A, Shin, MH & Lee, K 2016, 'Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis', Experimental dermatology, vol. 25, no. 11, pp. 880-886. https://doi.org/10.1111/exd.13111

Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis. / Noh, Ji Yeon; Shin, Jung U.; Park, Chang Ook; Lee, Nara; Jin, Shan; Kim, Seo Hyeong; Kim, Ji Hye; Min, Arim; Shin, Myeong Heon; Lee, Kwanghoon.

In: Experimental dermatology, Vol. 25, No. 11, 01.11.2016, p. 880-886.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis

AU - Noh, Ji Yeon

AU - Shin, Jung U.

AU - Park, Chang Ook

AU - Lee, Nara

AU - Jin, Shan

AU - Kim, Seo Hyeong

AU - Kim, Ji Hye

AU - Min, Arim

AU - Shin, Myeong Heon

AU - Lee, Kwanghoon

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein L-plastin and confirmed upregulation of L-plastin and p-L- plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of L-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of L-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of L-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-L- plastin as a potential drug target for eosinophil-targeted allergy therapy.

AB - Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein L-plastin and confirmed upregulation of L-plastin and p-L- plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of L-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of L-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of L-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-L- plastin as a potential drug target for eosinophil-targeted allergy therapy.

UR - http://www.scopus.com/inward/record.url?scp=85032698581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032698581&partnerID=8YFLogxK

U2 - 10.1111/exd.13111

DO - 10.1111/exd.13111

M3 - Article

C2 - 27304220

AN - SCOPUS:85032698581

VL - 25

SP - 880

EP - 886

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 11

ER -