Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis

Ji Yeon Noh, Jung U. Shin, Chang Ook Park, Nara Lee, Shan Jin, Seo Hyeong Kim, Ji Hye Kim, Arim Min, Myeong Heon Shin, Kwang Hoon Lee

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9 Citations (Scopus)

Abstract

Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein L-plastin and confirmed upregulation of L-plastin and p-L- plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of L-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of L-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of L-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-L- plastin as a potential drug target for eosinophil-targeted allergy therapy.

Original languageEnglish
Pages (from-to)880-886
Number of pages7
JournalExperimental dermatology
Volume25
Issue number11
DOIs
Publication statusPublished - 2016 Nov 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

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    Noh, J. Y., Shin, J. U., Park, C. O., Lee, N., Jin, S., Kim, S. H., Kim, J. H., Min, A., Shin, M. H., & Lee, K. H. (2016). Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of L-plastin in atopic dermatitis. Experimental dermatology, 25(11), 880-886. https://doi.org/10.1111/exd.13111