Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein L-plastin and confirmed upregulation of L-plastin and p-L- plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of L-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of L-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of L-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-L- plastin as a potential drug target for eosinophil-targeted allergy therapy.
Bibliographical noteFunding Information:
This study was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (2011-0016636) and a grant of the Korea Health Technology R&? Project through the Korea Health Industry ?evelopment Institute (KHI?I), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324).
This study was supported by the Basic Science Research Program of the National Research Foundation of Korea 501100003725 (NRF), funded by the MEST Ministry of Education, Science and Technology (2011-0016636) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute 501100003710 (KHIDI), funded by the MOHW Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324).
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
All Science Journal Classification (ASJC) codes
- Molecular Biology