Time course study of cytokine mRNA expression in LPS-stimulated porcine alveolar macrophages.

In Soo Choi, Na Ri Shin, Sung Jae Shin, Deog Yong Lee, Young Wook Cho, Han Sang Yoo

Research output: Contribution to journalArticle

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Abstract

The kinetics of cytokine mRNA expression was studied in porcine alveolar macrophages using an RT-PCR assay. The expression levels of IFN- gamma, IL-2, IL-4, IL-6, GM-CSF, IL-12 p35, and IL-12 p40 were examined after 2, 4, 14, 24, 48, and 72 h of incubation in unstimulated control and LPS-stimulated cells. The expression contents of IFN-gamma, IL-2, and IL-4 were not detected in both unstimulated and LPS-stimulated cells. On the other hand, the expression levels of IL-6, GM-CSF, and IL-12 in LPS-stimulated cells were almost always higher than those in control cells. Among those cytokines, IL-6 exhibited the predominant expression, and GM-CSF, IL-12 p40, and IL-12 p35 followed in the descending order. The times to reach the peak expression levels for IL-6, and GM-CSF, IL-12 p35, and IL-12 p40 were 14, and 24 h, respectively. After reaching the peak expression point, the expression levels of IL-6, GM-CSF, and IL-12 p40 reduced to the baseline by 72 h after stimulation, however, IL-12 p35 still kept a substantial expression by the same time. This study demonstrates that porcine alveolar macrophages primarily respond to express IL-6, GM-CSF, and IL-12 by LPS-stimulation and have a cytokine-specific expression profile during the stimulation time.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalJournal of veterinary science (Suwon-si, Korea)
Volume3
Issue number2
Publication statusPublished - 2002 Jun

Fingerprint

interleukin-12
Alveolar Macrophages
Interleukin-12
macrophages
cytokines
Swine
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Messenger RNA
swine
interleukin-6
Interleukin-6
interleukin-4
interleukin-2
Interleukin-4
Interleukin-2
cells
reverse transcriptase polymerase chain reaction
kinetics
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Choi, In Soo ; Shin, Na Ri ; Shin, Sung Jae ; Lee, Deog Yong ; Cho, Young Wook ; Yoo, Han Sang. / Time course study of cytokine mRNA expression in LPS-stimulated porcine alveolar macrophages. In: Journal of veterinary science (Suwon-si, Korea). 2002 ; Vol. 3, No. 2. pp. 97-102.
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Time course study of cytokine mRNA expression in LPS-stimulated porcine alveolar macrophages. / Choi, In Soo; Shin, Na Ri; Shin, Sung Jae; Lee, Deog Yong; Cho, Young Wook; Yoo, Han Sang.

In: Journal of veterinary science (Suwon-si, Korea), Vol. 3, No. 2, 06.2002, p. 97-102.

Research output: Contribution to journalArticle

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AU - Choi, In Soo

AU - Shin, Na Ri

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AU - Yoo, Han Sang

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