Time-lapse live-cell imaging reveals dual function of oseg4, drosophila WDR35, in ciliary protein trafficking

Nayoung Lee, Jina Park, Yong Chul Bae, Jung Ho Lee, Chul Hoon Kim, Seok Jun Moon

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Cilia are highly specialized antennae-like organelles that extend from the cell surface and act as cell signaling hubs. Intraflagellar transport (IFT) is a specialized form of intracellular protein trafficking that is required for the assembly and maintenance of cilia. Because cilia are so important, mutations in several IFT components lead to human disease. Thus, clarifying the molecular functions of the IFT proteins is a high priority in cilia biology. Live imaging in various species and cellular preparations has proven to be an important technique in both the discovery of IFT and the mechanisms by which it functions. Live imaging of Drosophila cilia, however, has not yet been reported. Here, we have visualized the movement of IFT in Drosophila cilia using time-lapse live imaging for the first time. We found that NOMPB-GFP (IFT88) moves according to distinct parameters depending on the ciliary segment. NOMPB-GFP moves at a similar speed in proximal and distal cilia toward the tip (~0.45 μm/s). As it returns to the ciliary base, however, NOMPB-GFP moves at ~0.12 μm/s in distal cilia, accelerating to ~0.70 μm/s in proximal cilia. Furthermore, while live imaging NOMPB-GFP, we observed one of the IFT proteins required for retrograde movement, Oseg4 (WDR35), is also required for anterograde movement in distal cilia. We anticipate our time-lapse live imaging analysis technique in Drosophila cilia will be a good starting point for a more sophisticated analysis of IFT and its molecular mechanisms.

Original languageEnglish
Pages (from-to)676-683
Number of pages8
JournalMolecules and cells
Volume41
Issue number7
DOIs
Publication statusPublished - 2018

Bibliographical note

Funding Information:
We thank the Bloomington Stock Center and Dr. M. Kernan for fly stocks. We thank the Korean Drosophila Resource Center for the generation of transgenic flies. We thank the Yonsei Advanced Imaging Center in cooperation with Carl Zeiss Microscopy, Yonsei University College of Medicine, for technical assistance. We thank the Drosophila Genomics Resource Center, supported by NIH grant 2P40OD010949 for Oseg4 cDNA. This work was supported by a National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (NRF-2016R1A5A2008630 and NRF-2018R1A2B3001668).

Publisher Copyright:
© 2018, Korean Society for Molecular and Cellular Biology. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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